@article{Yu:2020:10.1128/jcm.00843-20,
author = {Yu, L-S and Rodriguez-Manzano, J and Moser, N and Moniri, A and Malpartida-Cardenas, K and Miscourides, N and Sewell, T and Kochina, T and Brackin, A and Rhodes, J and Holmes, AH and Fisher, MC and Georgiou, P},
doi = {10.1128/jcm.00843-20},
journal = {Journal of Clinical Microbiology},
pages = {1--11},
title = {Rapid detection of azole-resistant Aspergillus fumigatus in clinical and environmental isolates using lab-on-a-chip diagnostic system},
url = {http://dx.doi.org/10.1128/jcm.00843-20},
volume = {58},
year = {2020}
}

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TY  - JOUR
AB  - Aspergillus fumigatus has widely evolved resistance to the most commonly used class of antifungal chemicals, the azoles. Current methods for identifying azole resistance are time-consuming and depend on specialized laboratories. There is an urgent need for rapid detection of these emerging pathogens at point-of-care to provide the appropriate treatment in the clinic and to improve management of environmental reservoirs to mitigate the spread of antifungal resistance. Our study demonstrates the rapid and portable detection of the two most relevant genetic markers linked to azole resistance, the mutations TR34 and TR46, found in the promoter region of the gene encoding the azole target, cyp51A. We developed a lab-on-a-chip platform consisting of: (1) tandem-repeat loop-mediated isothermal amplification, (2) state-of-the-art complementary metal-oxide-semiconductor microchip technology for nucleic-acid amplification detection and, (3) and a smartphone application for data acquisition, visualization and cloud connectivity. Specific and sensitive detection was validated with isolates from clinical and environmental samples from 6 countries across 5 continents, showing a lower limit-of-detection of 10 genomic copies per reaction in less than 30 minutes. When fully integrated with a sample preparation module, this diagnostic system will enable the detection of this ubiquitous fungus at the point-of-care, and could help to improve clinical decision making, infection control and epidemiological surveillance.
AU  - Yu,L-S
AU  - Rodriguez-Manzano,J
AU  - Moser,N
AU  - Moniri,A
AU  - Malpartida-Cardenas,K
AU  - Miscourides,N
AU  - Sewell,T
AU  - Kochina,T
AU  - Brackin,A
AU  - Rhodes,J
AU  - Holmes,AH
AU  - Fisher,MC
AU  - Georgiou,P
DO  - 10.1128/jcm.00843-20
EP  - 11
PY  - 2020///
SN  - 0095-1137
SP  - 1
TI  - Rapid detection of azole-resistant Aspergillus fumigatus in clinical and environmental isolates using lab-on-a-chip diagnostic system
T2  - Journal of Clinical Microbiology
UR  - http://dx.doi.org/10.1128/jcm.00843-20
UR  - https://jcm.asm.org/content/58/11/e00843-20
UR  - http://hdl.handle.net/10044/1/82429
VL  - 58
ER  - 

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