@article{Liu:2014:nar/gku080,
author = {Liu, B and Shadrin, A and Sheppard, C and Mekler, V and Xu, Y and Severinov, K and Matthews, S and Wigneshweraraj, S},
doi = {nar/gku080},
journal = {Nucleic Acids Research},
pages = {4294--4305},
title = {A bacteriophage transcription regulator inhibits bacterial transcription initiation by Sigma-factor displacement},
url = {http://dx.doi.org/10.1093/nar/gku080},
volume = {42},
year = {2014}
}

Download

TY  - JOUR
AB  - Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic β and β’ subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the σ70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.
AU  - Liu,B
AU  - Shadrin,A
AU  - Sheppard,C
AU  - Mekler,V
AU  - Xu,Y
AU  - Severinov,K
AU  - Matthews,S
AU  - Wigneshweraraj,S
DO  - nar/gku080
EP  - 4305
PY  - 2014///
SN  - 0305-1048
SP  - 4294
TI  - A bacteriophage transcription regulator inhibits bacterial transcription initiation by Sigma-factor displacement
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gku080
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000334761100024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://academic.oup.com/nar/article/42/7/4294/2437423
UR  - http://hdl.handle.net/10044/1/17928
VL  - 42
ER  - 

Download