@article{Yusuf:2015:10.1074/jbc.M115.637694,
author = {Yusuf, NA and Green, JL and Wall, RJ and Knuepfer, E and Moon, RW and Schulte-Huxel, C and Stanway, RR and Martin, SR and Howell, SA and Douse, CH and Cota, E and Tate, EW and Tewari, R and Holder, AA},
doi = {10.1074/jbc.M115.637694},
journal = {Journal of Biological Chemistry},
pages = {12147--12164},
title = {The Plasmodium Class XIV Myosin, MyoB, Has a Distinct Subcellular Location in Invasive and Motile Stages of the Malaria Parasite and an Unusual Light Chain},
url = {http://dx.doi.org/10.1074/jbc.M115.637694},
volume = {290},
year = {2015}
}

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TY  - JOUR
AB  - Myosin B (MyoB) is one of the two short class XIV myosinsencoded in the Plasmodium genome. Class XIV myosins arecharacterized by a catalytic “head,” a modified “neck,” and theabsence of a “tail” region. Myosin A (MyoA), the other class XIVmyosin in Plasmodium, has been established as a component ofthe glideosome complex important in motility and cell invasion,but MyoB is not well characterized. We analyzed the propertiesof MyoB using three parasite species as follows: Plasmodiumfalciparum, Plasmodium berghei, and Plasmodium knowlesi.MyoB is expressed in all invasive stages (merozoites, ookinetes,and sporozoites) of the life cycle, and the protein is found in adiscrete apical location in these polarized cells. In P. falciparum,MyoB is synthesized very late in schizogony/merogony, and itslocation in merozoites is distinct from, and anterior to, that of arange of known proteins present in the rhoptries, rhoptry neckor micronemes. Unlike MyoA, MyoB is not associated withglideosome complex proteins, including the MyoA light chain,myosin A tail domain-interacting protein (MTIP). A uniqueMyoB light chain (MLC-B) was identified that contains a calmodulin-likedomain at the C terminus and an extended N-terminalregion. MLC-B localizes to the same extreme apical polein the cell as MyoB, and the two proteins form a complex. Wepropose that MLC-B is a MyoB-specific light chain, and for theshort class XIV myosins that lack a tail region, the atypical myosinlight chains may fulfill that role.
AU  - Yusuf,NA
AU  - Green,JL
AU  - Wall,RJ
AU  - Knuepfer,E
AU  - Moon,RW
AU  - Schulte-Huxel,C
AU  - Stanway,RR
AU  - Martin,SR
AU  - Howell,SA
AU  - Douse,CH
AU  - Cota,E
AU  - Tate,EW
AU  - Tewari,R
AU  - Holder,AA
DO  - 10.1074/jbc.M115.637694
EP  - 12164
PY  - 2015///
SN  - 1083-351X
SP  - 12147
TI  - The Plasmodium Class XIV Myosin, MyoB, Has a Distinct Subcellular Location in Invasive and Motile Stages of the Malaria Parasite and an Unusual Light Chain
T2  - Journal of Biological Chemistry
UR  - http://dx.doi.org/10.1074/jbc.M115.637694
UR  - http://hdl.handle.net/10044/1/26872
VL  - 290
ER  - 

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