@article{Berry:2016:10.1016/j.str.2016.04.001,
author = {Berry, J and Xu, Y and Ward, PN and Lea, SM and Matthews, SJ and Pelicic, V},
doi = {10.1016/j.str.2016.04.001},
journal = {Structure},
pages = {926--934},
title = {A comparative structure/function analysis of two type IV pilin DNA receptors defines a novel mode of DNA-binding},
url = {http://dx.doi.org/10.1016/j.str.2016.04.001},
volume = {24},
year = {2016}
}

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TY  - JOUR
AB  - DNA transformation is a widespread process allowing bacteria to capture free DNAby using filamentous nano-machines composed of type IV pilins. These proteins canact as DNA receptors as demonstrated by the finding that Neisseria meningitidisComP minor pilin has intrinsic DNA-binding ability. ComP binds DNA better when itcontains the DNA uptake sequence (DUS) motif abundant in this species genome,playing a role in its trademark ability to selectively take up its own DNA. Here, wereport high-resolution structures for meningococcal ComP and Neisseria subflavaComPsub, which recognize different DUS motifs. We show that they are structurallyidentical type IV pilins that pack readily into filament models and display a uniqueDD-region delimited by two disulfide bonds. Functional analysis of ComPsub defines anew mode of DNA-binding involving the DD-region, adapted for exported DNAreceptors.
AU  - Berry,J
AU  - Xu,Y
AU  - Ward,PN
AU  - Lea,SM
AU  - Matthews,SJ
AU  - Pelicic,V
DO  - 10.1016/j.str.2016.04.001
EP  - 934
PY  - 2016///
SN  - 1878-4186
SP  - 926
TI  - A comparative structure/function analysis of two type IV pilin DNA receptors defines a novel mode of DNA-binding
T2  - Structure
UR  - http://dx.doi.org/10.1016/j.str.2016.04.001
UR  - http://hdl.handle.net/10044/1/31125
VL  - 24
ER  - 

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