@article{Holmes:2016:10.1038/srep27636,
author = {Holmes, AH and Gill, SK and Hui, K and Farne, H and Garnett, JP and Baines, DL and Moore, LSP and Filloux, A and Tregoning, JS},
doi = {10.1038/srep27636},
journal = {Scientific Reports},
title = {Increased airway glucose increases airway bacterial load in hyperglycaemia},
url = {http://dx.doi.org/10.1038/srep27636},
volume = {6},
year = {2016}
}

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TY  - JOUR
AB  - Diabetes is associated with increased frequency of hospitalization due to bacterial lung infection.We hypothesize that increased airway glucose caused by hyperglycaemia leads to increasedbacterial loads. In critical care patients, we observed that respiratory tract bacterial colonisationis significantly more likely when blood glucose is high. We engineered mutants in genesaffecting glucose uptake and metabolism (oprB, gltK, gtrS and glk) in Pseudomonas aeruginosa,strain PAO1. These mutants displayed attenuated growth in minimal medium supplemented withglucose as the sole carbon source. The effect of glucose on growth in vivo was tested usingstreptozocin-induced, hyperglycaemic mice, which have significantly greater airway glucose.Bacterial burden in hyperglycaemic animals was greater than control animals when infected withwild type but not mutant PAO1. Metformin pre-treatment of hyperglycaemic animals reducedboth airway glucose and bacterial load. These data support airway glucose as a criticaldeterminant of increased bacterial load during diabetes.
AU  - Holmes,AH
AU  - Gill,SK
AU  - Hui,K
AU  - Farne,H
AU  - Garnett,JP
AU  - Baines,DL
AU  - Moore,LSP
AU  - Filloux,A
AU  - Tregoning,JS
DO  - 10.1038/srep27636
PY  - 2016///
SN  - 2045-2322
TI  - Increased airway glucose increases airway bacterial load in hyperglycaemia
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep27636
UR  - http://hdl.handle.net/10044/1/32918
VL  - 6
ER  - 

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