The Chemistry Mass Spectrometry facility is housed at the new Imperial West Campus at White City in the Molecular Sciences Research Hub and has a range of Mass Spectrometers for a number of different applications.

Equipment

Micromass Autospec Premier/Agilent HP6890 GC

  • Ionistation Techniques: Electron Ionisation (EI), Chemical Ionisation (CI), Liquid Secondary Ion (LSIMS or FAB).  Mass Range: 50-700 Da (EI/CI), 20-2500 Da (LSIMS).  Provides both low and high resolution data.
  • CI and LSIMS run in both positive and negative modes.
  • GCMS provided in EI mode only.

Micromass MALDI-ToF

  • Mass Range: 500 Da->200 KDa in both positive and negative modes.  Low resolution data only.

Thermo Scientific Q-Exactive/Dionex Ultimate 3000/Easy NanoLC

  • Q-Exactive/Dionex Ultimate 3000: Ionisation: Electrospray (ES) and Atmospheric Pressure Chemical Ionisation (APCI), UHPLC-MS with PDA.
  • Q-Exactive/EasyNanoLC: Ionisation: Electrospray (ES), with EasyNano Source and EasyNano Columns for proteomic analysis.
  • Ionisation: Electrospray (ES). Mass Range: 100-2000 Da (mass range can be increased if necessary). Provides both low and high resolution data (high resolution data on singly charged ions up to 1500 Da) in both positive and negative modes.

Waters LCT Premier (ES-ToF)/Acquity i-Class

  • Ionisation: Electrospray (ES).  Mass Range: 100-2000 Da (mass range can be increased if necessary).  Provides both low and high resolution data (high resolution data on singly charged ions up to 1500 Da) in both positive and negative modes.
  • Direct Infusion or UPLC-MS analysis, with PDA.

DART probe

  • Used with LCT Premier.

Waters Acquity UPC2/SQD

  • Convergence Chromatography, uses both gases and liquids for UPLC-MS.  Single Quadrupole Mass Spectrometer, low resolution only in both positive and negative modes.

Mettler Toledo TGA/DSC 1LF/UMX, HIDEN ANALYTICAL HPR20- QIC Evolved gas mass spec, Thermo Nicolet iS10 FTIR

  • Thermal Analysis with IR and Mass Spec (up to 300 Da).

WATERS XEVO TQ-MS/ACQUITY UPLC

  • Ionisation: Electrospray (ES).  Mass Range 100-2000 Da
  • Positive and Negative low resolution data.
  • Triple Quad for fragmentation.
  • UPLC with UV

AGILENT 6125 MSD/1260 UHPLC

  • Ionisation: Electrospray (ES).  Mass Range to 1200 Da.
  • Postive and Negative low resolution data.

AGILENT INTUVO 9000 GC WITH 5977 MSD (HIGH EFFICIENCY SOURCE)

  • Low resolution GC-EI data
  • Mass range up to 1000 Da

How to use the Mass Spectrometry Facility

(Imperial College Only)

In order to use the Chemistry Mass Spectrometry facility you must be registered as a 'User' on Sharepoint. To register please send the following information to l.haigh@imperial.ac.uk

  • Department
  • Supervisor
  • Cost Centre
  • Activity Code (must be approved by your supervisor)
  • You must also have a valid Imperial College e-mail address

Once registered, you will receive a link to the Sharepoint online booking system and please see the Submission Guide document (below) on how to complete the online form.

Please note: You must be using a college computer and Internet Explorer is preferred to access Sharepoint.  Registration may take up to 72 hours.

Charges

AS OF FEBRUARY 1ST 2018

  • Imperial Users Non FEC: £7.00 per sample
  • Imperial Users FEC: £18.00 per sample
  • External Users: POA

Open-Access Equipment/Training

There are five open-access instruments available:

  • Waters Xevo TQ-MS/Acquity UPLC
  • Agilent 6125 MS/1260 LC
  • Micromass MALDI-ToF
  • Agilent Intuvo 9000 GC/5977 MSD
  • Mettler Toledo TGA/DSC 1LF/UMX, HIDEN HPR20- QIC EGA, Thermo Nicolet iS10 FTIR

In order to use the open-access facility you must already be registered to the Sharepoint site and attend a training course for each piece of equipment.  There is a 'request for training' option available on the Mass Spec sharepoint booking site.  Once requested, you will receive an email with a date and time for training, please confirm whether or not you can attend.  Training courses take 1-2 hours and include a safety induction.   Training open to MRes, PhD status and above.

If you just want more information on how LCMS/MALDI works etc, or not sure if you want/need to run your own samples?  Please email Lisa to book yourself on a 'get-to-know' session, which covers hardware, troubleshooting, hints and tips.  This is open to all registered users of the Mass Spec Facility.

Contact Details

To register as a User of the Mass Spectrometry Facility and for all other enquiries (external work considered) please contact:

Dr. Lisa D. Haigh (Mass Spectrometry Service Manager)
e-mail (preferred): l.haigh@imperial.ac.uk
Tel: +44 (0) 207 594 5748