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• JOURNAL ARTICLE
  Watson T, Andrews N, Davis S, Bugeon L, Dallman MD, McGinty Jet al., 2017,

  OPTiM: Optical projection tomography integrated microscope using open-source hardware and software

  , PLOS ONE, Vol: 12, ISSN: 1932-6203
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• CONFERENCE PAPER
  Jha A, Progatzky F, Wane M, Thwaites RS, McBrien M, Brimley J, Tunstall T, Shattock RJ, Bugeon L, Openshaw PJM, Dallman MJ, Hansel TTet al., 2016,

  Human nasal mucosal responses to TLR agonists are mirrored by the zebrafish gill

  , British Association of Lung Research Summer Congress

  Introduction: There are few reliable ways to study respiratory mucosal immune responses to viruses, viral-type toll-like receptor (TLR) agonists and vaccines. To investigate innate immune responses to TLR agonists (TLR3: poly IC/ poly ICLC; TLR7/8: resiquimod), we compared the effects on human nasal mucosa and zebrafish gills in vivo. Methods: Nasal challenge of adult volunteers was performed with saline, poly IC (n=4), poly ICLC (n=4) or resiquimod (n=8; 5 non-atopic, 3 atopic). Nasal mucosal lining fluid (MLF) was obtained by nasosorption at regular intervals up to 24 hours after challenge; nasal obstruction was monitored by peak nasal inspiratory flow (PNIF) and total nasal symptom scores (TNSS). Cytokines and interferons were measured in MLF using electrochemiluminescence on the Meso Scale Discovery (MSD) platform. Adult zebrafish gills were exposed to the same TLR agonists and gene expression was quantified in gill tissue at similar time-points. Results: Nasal challenge with TLR3 agonists failed to elicit any significant responses when compared to saline. In contrast resiquimod (10μg/100μl per nostril) caused a potent induction of cytokines with an early release (1-3 hours) of IFN-α2a, TNF-α and IL-1β and a later release (after 4 hours) of IFN-γ. The 3 volunteers with the highest levels of IFN-α2a were atopic. Six volunteers were asymptomatic and two volunteers had flu-like symptoms. There were no significant changes in clinical correlates of nasal obstruction. After resiquimod administration, but not TLR3 agonists, zebrafish gills showed an immune profile remarkably analogous to human nasal responses. Conclusion: The TLR7/8 agonist resiquimod is a potent mucosal inducer of IFN-α2a, IFN-γ and proinflammatory cytokines, whilst TLR3 agonists failed to stimulate mucosal innate immune responses. Zebrafish gills accurately mimic human nasal mucosal responses following exposure to TLR agonists, offering translational app

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• JOURNAL ARTICLE
  Kumar S, Lockwood N, Ramel M-C, Correia T, Ellis M, Alexandrov Y, Andrews N, Patel R, Bugeon L, Dallman MJ, Brandner S, Arridge S, Katan M, McGinty J, Frankel P, French PMWet al., 2016,

  Quantitative in vivo optical tomography of cancer progression & vasculature development in adult zebrafish

  , ONCOTARGET, Vol: 7, Pages: 43939-43948, ISSN: 1949-2553
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  • Citations: 3
• JOURNAL ARTICLE
  Andrews N, Ramel M-C, Kumar S, Alexandrov Y, Kelly DJ, Warren SC, Kerry L, Lockwood N, Frolov A, Frankel P, Bugeon L, McGinty J, Dallman MJ, French PMWet al., 2016,

  Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time

  , JOURNAL OF BIOPHOTONICS, Vol: 9, Pages: 414-424, ISSN: 1864-063X
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  • Citations: 11
• JOURNAL ARTICLE
  Progatzky F, Cook HT, Lamb JR, Bugeon L, Dallman MJet al., 2016,

  Mucosal inflammation at the respiratory interface: a zebrafish model

  , AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 310, Pages: L551-L561, ISSN: 1040-0605
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  • Citations: 6
• JOURNAL ARTICLE
  Jones PJM, Sim A, Taylor HB, Bugeon L, Dallman MJ, Pereira B, Stumpf MPH, Liepe Jet al., 2015,

  Inference of random walk models to describe leukocyte migration

  , PHYSICAL BIOLOGY, Vol: 12, ISSN: 1478-3967
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  • Citations: 9
• JOURNAL ARTICLE
  Broncel M, Serwa RA, Ciepla P, Krause E, Dallman MJ, Magee AI, Tate EWet al., 2015,

  Myristoylation profiling in human cells and zebrafish

  , DATA IN BRIEF, Vol: 4, Pages: 379-383, ISSN: 2352-3409
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  • Citations: 3
• JOURNAL ARTICLE
  Correia T, Lockwood N, Kumar S, Yin J, Ramel M-C, Andrews N, Katan M, Bugeon L, Dallman MJ, McGinty J, Frankel P, French PMW, Arridge Set al., 2015,

  Accelerated Optical Projection Tomography Applied to In Vivo Imaging of Zebrafish

  , PLOS ONE, Vol: 10, ISSN: 1932-6203
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  • Citations: 11
• JOURNAL ARTICLE
  Broncel M, Serwa RA, Ciepla P, Krause E, Dallman MJ, Magee AI, Tate EWet al., 2015,

  Multifunctional Reagents for Quantitative Proteome-Wide Analysis of Protein Modification in Human Cells and Dynamic Profiling of Protein Lipidation During Vertebrate Development

  , ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 54, Pages: 5948-5951, ISSN: 1433-7851
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  • Citations: 31
• JOURNAL ARTICLE
  Chen L, Alexandrov Y, Kumar S, Andrews N, Dallman MJ, French PMW, McGinty Jet al., 2015,

  Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography

  , BIOMEDICAL OPTICS EXPRESS, Vol: 6, Pages: 1253-1261, ISSN: 2156-7085
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  • Citations: 2
• JOURNAL ARTICLE
  Progatzky F, Sangha NJ, Yoshida N, McBrien M, Cheung J, Shia A, Scott J, Marchesi JR, Lamb JR, Bugeon L, Dallman MJet al., 2014,

  Dietary cholesterol directly induces acute inflammasome-dependent intestinal inflammation

  , NATURE COMMUNICATIONS, Vol: 5, ISSN: 2041-1723
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  • Citations: 32
• CONFERENCE PAPER
  Progatzky F, Sangha NJ, Yoshida N, McBrien M, Cheung J, Shia A, Scott J, Marchesi JR, Lamb JR, Bugeon L, Dallman MJet al., 2014,

  Intestinal inflammation induced by dietary cholesterol in Zebrafish

  , 9th European-Mucosal-Immunology-Group Meeting, Publisher: WILEY-BLACKWELL, Pages: 27-28, ISSN: 0019-2805
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• JOURNAL ARTICLE
  Chen L, Kumar S, Kelly D, Andrews N, Dallman MJ, French PMW, McGinty Jet al., 2014,

  Remote focal scanning optical projection tomography with an electrically tunable lens

  , BIOMEDICAL OPTICS EXPRESS, Vol: 5, Pages: 3367-3375, ISSN: 2156-7085
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  • Citations: 9
• JOURNAL ARTICLE
  Ciepla P, Konitsiotis AD, Serwa RA, Masumoto N, Leong WP, Dallman MJ, Magee AI, Tate EWet al., 2014,

  New chemical probes targeting cholesterylation of Sonic Hedgehog in human cells and zebrafish

  , Chemical Science, Vol: 5, Pages: 4249-4259, ISSN: 2041-6520

  Sonic Hedgehog protein (Shh) is a morphogen molecule important in embryonic development and in theprogression of many cancer types in which it is aberrantly overexpressed. Fully mature Shh requiresattachment of cholesterol and palmitic acid to its C- and N-termini, respectively. The study of lipidatedShh has been challenging due to the limited array of tools available, and the roles of theseposttranslational modifications are poorly understood. Herein, we describe the development andvalidation of optimised alkynyl sterol probes that efficiently tag Shh cholesterylation and enable itsvisualisation and analysis through bioorthogonal ligation to reporters. An optimised probe was shown tobe an excellent cholesterol biomimetic in the context of Shh, enabling appropriate release of tagged Shhfrom signalling cells, formation of multimeric transport complexes and signalling. We have used thisprobe to determine the size of transport complexes of lipidated Shh in culture medium and expressionlevels of endogenous lipidated Shh in pancreatic ductal adenocarcinoma cell lines through quantitativechemical proteomics, as well as direct visualisation of the probe by fluorescence microscopy anddetection of cholesterylated Hedgehog protein in developing zebrafish embryos. These sterol probesprovide a set of novel and well-validated tools that can be used to investigate the role of lipidation onactivity of Shh, and potentially other members of the Hedgehog protein family

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• JOURNAL ARTICLE
  Progatzky F, Dallman MJ, Lo Celso C, 2013,

  From seeing to believing: labelling strategies for in vivo cell-tracking experiments

  , INTERFACE FOCUS, Vol: 3, ISSN: 2042-8898
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  • Citations: 95
• JOURNAL ARTICLE
  Taylor HB, Liepe J, Barthen C, Bugeon L, Huvet M, Kirk PDW, Brown SB, Lamb JR, Stumpf MPH, Dallman MJet al., 2013,

  P38 and JNK have opposing effects on persistence of in vivo leukocyte migration in zebrafish

  , IMMUNOLOGY AND CELL BIOLOGY, Vol: 91, Pages: 60-69, ISSN: 0818-9641
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  • Citations: 8
• JOURNAL ARTICLE
  Le Friec G, Sheppard D, Whiteman P, Karsten CM, Shamoun SA-T, Laing A, Bugeon L, Dallman MJ, Melchionna T, Chillakuri C, Smith RA, Drouet C, Couzi L, Fremeaux-Bacchi V, Koehl J, Waddington SN, McDonnell JM, Baker A, Handford PA, Lea SM, Kemper Cet al., 2012,

  The CD46-Jagged1 interaction is critical for human T(H)1 immunity

  , NATURE IMMUNOLOGY, Vol: 13, Pages: 1213-+, ISSN: 1529-2908
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  • Citations: 75
• CONFERENCE PAPER
  Le Friec G, Shamoun S, Couzi L, Fremeaux-Bacchi V, Baker A, Bugeon L, Dallman M, Handford P, Lea S, Kemper Cet al., 2012,

  Jagged1 is a CD46 ligand and disturbance in CD46/Jagged 1 interaction leads to abnormal Th1 function and infections in humans

  , European Congress of Immunology, Publisher: WILEY-BLACKWELL, Pages: 8-8, ISSN: 0019-2805
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• JOURNAL ARTICLE
  Progatzky F, Taylor H, Bugeon L, Cassidy S, Radbruch A, Dallman MJ, Lamb JRet al., 2012,

  The role of Nfil3 in zebrafish hematopoiesis

  , DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, Vol: 38, Pages: 187-192, ISSN: 0145-305X
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  • Citations: 3
• JOURNAL ARTICLE
  Gentle ME, Rose A, Bugeon L, Dallman MJet al., 2012,

  Noncanonical Notch Signaling Modulates Cytokine Responses of Dendritic Cells to Inflammatory Stimuli

  , JOURNAL OF IMMUNOLOGY, Vol: 189, Pages: 1274-1284, ISSN: 0022-1767
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  • Citations: 24
• JOURNAL ARTICLE
  Rose A, Kay E, Wren BW, Dallman MJet al., 2012,

  The Campylobacter jejuni NCTC11168 capsule prevents excessive cytokine production by dendritic cells

  , MEDICAL MICROBIOLOGY AND IMMUNOLOGY, Vol: 201, Pages: 137-144, ISSN: 0300-8584
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  • Citations: 15
• JOURNAL ARTICLE
  Chen L, McGinty J, Taylor HB, Bugeon L, Lamb JR, Dallman MJ, French PMWet al., 2012,

  Incorporation of an experimentally determined MTF for spatial frequency filtering and deconvolution during optical projection tomography reconstruction

  , OPTICS EXPRESS, Vol: 20, Pages: 7323-7337, ISSN: 1094-4087
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  • Citations: 10
• JOURNAL ARTICLE
  Liepe J, Taylor H, Barnes CP, Huvet M, Bugeon L, Thorne T, Lamb JR, Dallman MJ, Stumpf MPHet al., 2012,

  Calibrating spatio-temporal models of leukocyte dynamics against in vivo live-imaging data using approximate Bayesian computation

  , INTEGRATIVE BIOLOGY, Vol: 4, Pages: 335-345, ISSN: 1757-9694
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  • Citations: 21
• JOURNAL ARTICLE
  Bugeon L, Taylor HB, Progatzky F, Lin MI, Ellis CD, Welsh N, Smith E, Vargesson N, Gray C, Renshaw SA, Chico TJA, Zon LI, Lamb J, Dallman MJet al., 2011,

  The NOTCH pathway contributes to cell fate decision in myelopoiesis

  , HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Vol: 96, Pages: 1753-1760, ISSN: 0390-6078
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  • Citations: 5
• JOURNAL ARTICLE
  Silk D, Kirk PDW, Barnes CP, Toni T, Rose A, Moon S, Dallman MJ, Stumpf MPHet al., 2011,

  Designing attractive models via automated identification of chaotic and oscillatory dynamical regimes

  , NATURE COMMUNICATIONS, Vol: 2, ISSN: 2041-1723
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  • Citations: 18
• JOURNAL ARTICLE
  Lara R, Mauri FA, Taylor H, Derua R, Shia A, Gray C, Nicols A, Shiner RJ, Schofield E, Bates PA, Waelkens E, Dallman M, Lamb J, Zicha D, Downward J, Seckl MJ, Pardo OEet al., 2011,

  An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis

  , ONCOGENE, Vol: 30, Pages: 3513-3521, ISSN: 0950-9232
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  • Citations: 57
• JOURNAL ARTICLE
  Pridgeon C, Bugeon L, Donnelly L, Straschil U, Tudhope SJ, Fenwick P, Lamb JR, Barnes PJ, Dallman MJet al., 2011,

  Regulation of IL-17 in chronic inflammation in the human lung

  , CLINICAL SCIENCE, Vol: 120, Pages: 515-524, ISSN: 0143-5221
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  • Citations: 30
• JOURNAL ARTICLE
  Lara R, Mauri F, Taylor H, Derua R, Shia A, Gray C, Nicols A, Shiner RJ, Schofield EE, Bates P, Waelkens E, Dallman M, Lamb J, Zicha D, Downward J, Seckl M, Pardo Oet al., 2011,

  IDENTIFICATION OF RSK1 AS A KEY MODULATOR OF LUNG CANCER METASTASIS

  , JOURNAL OF THORACIC ONCOLOGY, Vol: 6, Pages: S514-S515, ISSN: 1556-0864
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• JOURNAL ARTICLE
  McGinty J, Taylor HB, Chen L, Bugeon L, Lamb JR, Dallman MJ, French PMWet al., 2011,

  In vivo fluorescence lifetime optical projection tomography

  , BIOMEDICAL OPTICS EXPRESS, Vol: 2, Pages: 1340-1350, ISSN: 2156-7085
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  • Citations: 34
• JOURNAL ARTICLE
  Kumar S, Alibhai D, Margineanu A, Laine R, Kennedy G, McGinty J, Warren S, Kelly D, Alexandrov Y, Munro I, Talbot C, Stuckey DW, Kimberly C, Viellerobe B, Lacombe F, Lam EW-F, Taylor H, Dallman MJ, Stamp G, Murray EJ, Stuhmeier F, Sardini A, Katan M, Elson DS, Neil MAA, Dunsby C, French PMWet al., 2011,

  FLIM FRET Technology for Drug Discovery: Automated Multiwell-Plate High-Content Analysis, Multiplexed Readouts and Application in Situ

  , CHEMPHYSCHEM, Vol: 12, Pages: 609-626, ISSN: 1439-4235
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  • Citations: 40

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