Citation

BibTex format

@article{McGinty:2011:10.1364/BOE.2.001340,
author = {McGinty, J and Taylor, HB and Chen, L and Bugeon, L and Lamb, JR and Dallman, MJ and French, PMW},
doi = {10.1364/BOE.2.001340},
journal = {Biomedical Optics Express},
pages = {1340--1350},
title = {In vivo fluorescence lifetime optical projection tomography},
url = {http://dx.doi.org/10.1364/BOE.2.001340},
volume = {2},
year = {2011}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.
AU - McGinty,J
AU - Taylor,HB
AU - Chen,L
AU - Bugeon,L
AU - Lamb,JR
AU - Dallman,MJ
AU - French,PMW
DO - 10.1364/BOE.2.001340
EP - 1350
PY - 2011///
SN - 2156-7085
SP - 1340
TI - In vivo fluorescence lifetime optical projection tomography
T2 - Biomedical Optics Express
UR - http://dx.doi.org/10.1364/BOE.2.001340
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000299878000029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/69706
VL - 2
ER -