@inbook{Liu:2014:10.1007/978-4-431-54836-2_88-2,
author = {Liu, Y and Childs, RA and Feizi, T},
booktitle = {Glycoscience: Biology and Medicine},
doi = {10.1007/978-4-431-54836-2_88-2},
editor = {Endo and Seeberger and Hart and Wong and Taniguchi},
publisher = {Springer Japan},
title = {Neoglycolipid (NGL)-Based Glycan Microarray System for Ligand Discovery},
url = {http://dx.doi.org/10.1007/978-4-431-54836-2_88-2},
year = {2014}
}

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TY  - CHAP
AB  - It is now appreciated that carbohydrate–protein interactions are integral to many physiological processes and are directly or indirectly involved in the majority of disease processes, infective or noninfective, including cancer. Carbohydrate microarrays have emerged as powerful tools for elucidating the ligands involved in these interactions. However, as oligosaccharides cannot be cloned and expressed as with DNA and proteins, few laboratories have libraries of sequence-defined oligosaccharide probes with sufficient breadth to tackle the unraveling of diverse carbohydrate–protein interactions. Microarray screening analyses are offered to the scientific community by the Wellcome Trust-supported carbohydrate microarray facility in the Glycosciences Laboratory at Imperial College London and by the NIH-supported Consortium for Functional Glycomics. This chapter gives a brief account of a technology, the neoglycolipid (NGL) technology, first introduced in 1985 and converted into a glycan microarray system in 2002. Results are highlighted from analyses using this system, also including designer arrays, which entail microarrays of NGLs specifically derived from relevant ligand-bearing glycomes in order to reveal the oligosaccharide ligands they harbor and lead to their isolation and characterization. These include discoveries of new ligands in endogenous recognition and pathogen–host interactions and assignments of long-sought cancer-associated antigens.
AU  - Liu,Y
AU  - Childs,RA
AU  - Feizi,T
DO  - 10.1007/978-4-431-54836-2_88-2
PB  - Springer Japan
PY  - 2014///
SN  - 978-4-431-54836-2
TI  - Neoglycolipid (NGL)-Based Glycan Microarray System for Ligand Discovery
T1  - Glycoscience: Biology and Medicine
UR  - http://dx.doi.org/10.1007/978-4-431-54836-2_88-2
ER  - 

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