Citation

BibTex format

@inbook{Kis:2014,
author = {Kis, Z and Towhidi, L and Ip, H and Drakakis, E and Bharath, A and Krams, R},
booktitle = {Microarrays: Principles, Applications and Technologies},
pages = {49--67},
title = {An In situ electroporation and flow device for mechanotransduction studies},
year = {2014}
}

RIS format (EndNote, RefMan)

TY  - CHAP
AB - © 2014 by Nova Science Publishers, Inc. All rights reserved. The ability of cells to receive and respond to chemical and physical signals from beyond the plasma membrane is fundamental to life. Sensing and processing of stimuli (such as hormones, mechanical factor s, light, heat, neurotransmitters, nutrients, odorants, etc.) is mediated by signal-transduction pathways, molecular circuits that detect, amplify, and integrate diverse external signals to govern functions such as cellular survival, proliferation, differentiation, and apoptosis. These information-processing circuits that control intra- and inter- cellular activities at a molecular level are extremely widespread and complex. For example, approximately half of the 25 largest protein families encoded by the human genome, deal primarily with information processing. Characterizing signalling pathways would not only offer a better understanding of life at molecular level, but would also allow the treatment of diseases. To characterize these extremely complex signalling pathways, high throughput systems are required that can add functions (gain-of-function) and delete functions (lossof- function) from primary cells by inserting cDNA and/or siRNA, respectively. Furthermore, in order to mimic physiologically relevant conditions, such as blood flow, cells need to be exposed to biomechanical stimulus. Here, we describe the design and experimental verification of a device for applying biomechanical stimulus in the form of fluid shear stress and electroporation transfection of primary adherent cells. To that end, primary cells are seeded in an incubation chamber on a coated conductive glass slide allowing in situ electroporation to be taken place. Electroporation with our device on hard-to-transfect endothelial cells yields an siRNA transfection efficiency of 70% while keeping cell viability above 95%. Next, the microfluidics functionality of the platform was proven by deleting the inherent flow alignment functio
AU - Kis,Z
AU - Towhidi,L
AU - Ip,H
AU - Drakakis,E
AU - Bharath,A
AU - Krams,R
EP - 67
PY - 2014///
SN - 9781629487137
SP - 49
TI - An In situ electroporation and flow device for mechanotransduction studies
T1 - Microarrays: Principles, Applications and Technologies
ER -