Composite microscopic images taken using FILM microscopic equipment

The Zeiss Elyra PS1 features AxioObserver Z1 motorised inverted microscope transmitted light brightfield and DIC, with incident light fluorescence; an incubation system for long-term live cell studies, with both software controlled temperature regulation and CO2 regulation (Tokai); a CoolLED pE-4000 LED-based fluorescence source; as well as dual camera multi-channel imaging (dual channel simultaneous).


Advantages of this microscope

  • Total Internal Reflectance Microscopy (TIRFM)
  • Structured Illumination Microscopy  (SIM) - a widefield technique in which a grid pattern is generated through interference of diffraction orders and superimposed on the specimen while capturing images. The grid pattern is shifted or rotated in steps between the capture of each image set. High-frequency information can be extracted from the raw data to produce a reconstructed image having a lateral resolution approximately twice that of diffraction-limited instruments and an axial resolution ranging between 150 and 300 nanometers.
  • PhotoActivated Localization Microscopy (PALM)
  • Stochastic Optical Reconstruction Microscopy (STORM)

Location

Sir Alexander Fleming building, SAF 530


Quickstart guide

Quickstart guide - FILM SR1 Zeiss Elyra PS1 - Super Resolution TIRF (PDF)

Quickstart guide - FILM SR1 Zeiss Elyra PS1 Super Resolution - SIM(PDF)

Quickstart guide - FILM SR1 Zeiss Elyra PS1 Super Resolution - PALM/STORM/TIRF(P


Hardware configuration

Please see the FILM wiki page - FILM SR1 hardware configuration


Offline software