Composite microscopic images taken using FILM microscopic equipment

Inverted Widefield Microscope with LED illumination Zeiss Axio Observer inverted microscope with a fully motorised stage controlled by Zen acquisition software. This system is also equipped with a large range of filter sets, LED light sources from UV to far-red, and a highly sensitive Hamamatsu Flash 4 camera for fast, low-light imaging. Additionally, it is equipped with a colour camera for standard histology capable of tiling and stitching.


Advantages of this microscope

  • Multi-Channel, Z-Stack, Timelapse, Tiles & Positions 
  • Slide, dish and multiwell formats
  • Software autofocus
  • Deconvolution module
  • Lumencor LED light source and a range of filters for fast switching of excitation wavelengths
  • Live cell environmental control system including temperature and CO2 control
  • Hamamatsu Flash 4.0 fast camera
  • IC5 Colour camera

Location

Sir Alexander Fleming building, SAF 527


Quickstart guide

Quickstart guide - Widefield 3 Zeiss Cell observer ZEN (PDF)‌

WF3 Live Imaging Guide (PDF)

Widefield Tiling Method (PDF)

Sample Carrier Setup (PDF)

Multi-position acquisition (PDF)

Tiling using Colour Camera (PDF)

Stitching in ZEN Blue (PDF)


Hardware configuration

Please see the FILM wiki page - WF3 hardware configuration

LED and Filters specifications (PDF)

Frame Size Calculator (XLS)

Flash Camera Data (PDF)

Zeiss Objective List (PDF)


Offline software


General enquiries


FILM
Sir Alexander Fleming Building
South Kensington Campus
Imperial College London
Exhibition Road
London SW7 2AZ, UK

film-service@imperial.ac.uk