@article{Pollock:2016:10.1371/journal.pone.0146905,
author = {Pollock, KM and Montamat-Sicotte, D and Grass, L and Cooke, G and Kapembwa, M and Kon, O and Sampson, R and Taylor, G and Lalvani, A},
doi = {10.1371/journal.pone.0146905},
journal = {PLOS One},
title = {PD-1 expression and cytokine secretion profiles of Mycobacterium tuberculosis-specific CD4+ T-cell subsets; potential correlates of containment in HIV-TB co-infection},
url = {http://dx.doi.org/10.1371/journal.pone.0146905},
volume = {11},
year = {2016}
}

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TY  - JOUR
AB  - HIV co-infection is an important risk factor for tuberculosis (TB) providing a powerful model in which to dissect out defective, protective and dysfunctional Mycobacterium tuberculosis (MTB)-specific immune responses. To identify the changes induced by HIV co-infection we compared MTB-specific CD4+ responses in subjects with active TB and latent TB infection (LTBI), with and without HIV co-infection. CD4+ T-cell subsets producing interferon-gamma (IFN-γ), interleukin-2 (IL-2) and tumour necrosis factor-alpha (TNF-α) and expressing CD279 (PD-1) were measured using polychromatic flow-cytometry. HIV-TB co-infection was consistently and independently associated with a reduced frequency of CD4+ IFN-γ and IL-2-dual secreting T-cells and the proportion correlated inversely with HIV viral load (VL). The impact of HIV co-infection on this key MTB-specific T-cell subset identifies them as a potential correlate of mycobacterial immune containment. The percentage of MTB-specific IFN-γ-secreting T-cell subsets that expressed PD-1 was increased in active TB with HIV co-infection and correlated with VL. This identifies a novel correlate of dysregulated immunity to MTB, which may in part explain the paucity of inflammatory response in the face of mycobacterial dissemination that characterizes active TB with HIV co-infection.
AU  - Pollock,KM
AU  - Montamat-Sicotte,D
AU  - Grass,L
AU  - Cooke,G
AU  - Kapembwa,M
AU  - Kon,O
AU  - Sampson,R
AU  - Taylor,G
AU  - Lalvani,A
DO  - 10.1371/journal.pone.0146905
PY  - 2016///
SN  - 1932-6203
TI  - PD-1 expression and cytokine secretion profiles of Mycobacterium tuberculosis-specific CD4+ T-cell subsets; potential correlates of containment in HIV-TB co-infection
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0146905
UR  - http://hdl.handle.net/10044/1/28840
VL  - 11
ER  - 

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