@article{Salehi-Reyhani:2011,
author = {Salehi-Reyhani, A and Kaplinsky, J and Burgin, E and Novakova, M and deMello, AJ and Templer, RH and Parker, P and Neil, MAA and Ces, O and French, P and Willison, KR and Klug, D},
journal = {Lab Chip},
pages = {1256--1261},
title = {A first step towards practical single cell proteomics: a microfluidic antibodycapture chip with TIRF detection},
url = {http://hdl.handle.net/10044/1/10086},
volume = {11},
year = {2011}
}
TY - JOUR
AB - We have developed a generic platform to undertake the analysis of protein copy number from singlecells. The approach described here is ‘all-optical’ whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by laser-induced microcavitation, and the protein released from a single cell is measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device. The platform was tested using GFP transfected cells and the relative precision of the measurement method was determined to be 88%. Single cell measurements were also made on a breast cancer cell line to measure the relative levels of unlabelled human tumour suppressor protein p53 using a chip incorporating an antibody sandwich assay format. These results suggest that this is a viable method for measuring relative protein levels in single cells.
AU - Salehi-Reyhani,A
AU - Kaplinsky,J
AU - Burgin,E
AU - Novakova,M
AU - deMello,AJ
AU - Templer,RH
AU - Parker,P
AU - Neil,MAA
AU - Ces,O
AU - French,P
AU - Willison,KR
AU - Klug,D
EP - 1261
PY - 2011///
SP - 1256
TI - A first step towards practical single cell proteomics: a microfluidic antibodycapture chip with TIRF detection
T2 - Lab Chip
UR - http://hdl.handle.net/10044/1/10086
VL - 11
ER -