Citation

BibTex format

@inproceedings{Casey:2015,
author = {Casey, DR and Wylie, D and Gallo, J and Dent, MR and Salehi-Reyhani, A and Wilson, R and Brooks, NJ and Long, NJ and Willison, KR and Klug, DR and Neil, MAA and Neale, SL and Cooper, JM and Ces, O},
title = {A Novel, all-optical tool for controllable and non-destructive poration of cells with single-micron resolution},
url = {http://hdl.handle.net/10044/1/26458},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - CPAPER
AB - © OSA 2015. We demonstrate controllable poration within ≈1 μm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience. As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single- and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell's plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.
AU - Casey,DR
AU - Wylie,D
AU - Gallo,J
AU - Dent,MR
AU - Salehi-Reyhani,A
AU - Wilson,R
AU - Brooks,NJ
AU - Long,NJ
AU - Willison,KR
AU - Klug,DR
AU - Neil,MAA
AU - Neale,SL
AU - Cooper,JM
AU - Ces,O
PY - 2015///
TI - A Novel, all-optical tool for controllable and non-destructive poration of cells with single-micron resolution
UR - http://hdl.handle.net/10044/1/26458
ER -