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  • CONFERENCE PAPER
    Bernal P, Civantos C, Filloux A, Llamas MAet al.,

    Type VI secretion in the plant growth promoting rhizobacteria Pseudomonas putida

    , FEMS Microbiology Congress 2015

    BackgroundBacterial type VI secretion systems (T6SSs) are recently discovered nanomachines used to inject effectors into prokaryotic or eukaryotic cells. Therefore, T6SSs are involved in both inter-bacterial competition and bacterial pathogenesis.ObjectivesThe aim is the study of the T6SS of Pseudomonas putida a soil bacterium with the capacity to colonise the root of crop plants. The colonisation by this bacterium provides growth advantages to the plant and, importantly, protection against plant pathogens. This makes P. putida a relevant biocontrol agent. Since T6SS is mainly used by environmental bacteria for interbacterial competition, one might speculate that T6SSs play a relevant role in the biocontrol properties of P. putida. Methods• in silico analysis of P. putida KT2440 genome • Competition assays to determine H1-T6SS activity and for the identification of H1-T6SS targets.• Regulatory studies: qRT-PCR, transcriptional fusionsConclusionsThe in silico analysis has revealed the existence of three putative T6SSs (H1, H2, and H3). The clusters contain the genes encoding the conserved core components and some accessories, including regulatory proteins and toxins-immunity pairs. Additional T6SS-related genes are found scattered on the chromosome.By competition assays we have determined that H1-T6SS is active and that mutants in H1-T6SS structural components lack the ability to kill model prey strains. Moreover, the system can be used to kill serious phytopathogens such as Pseudomonas syringae in in vitro assays. Interestingly, the H1-T6SS is induced in stationary phase and controlled by the global regulators RetS and GacS-GacA, and by two alternative sigma factors, RpoS and RpoN.

  • JOURNAL ARTICLE
    Taylor JD, Matthews SJ,

    New insight into the molecular control of bacterial functional amyloids.

    , Frontiers in Cellular and Infection Microbiology, Vol: 5, ISSN: 2235-2988

    Amyloid protein structure has been discovered in a variety of functional or pathogenic contexts. What distinguishes the former from the latter is that functional amyloid systems possess dedicated molecular control systems that determine the timing, location, and structure of the fibers. Failure to guide this process can result in cytotoxicity, as observed in several pathologies like Alzheimer's and Parkinson's Disease. Many gram-negative bacteria produce an extracellular amyloid fiber known as curli via a multi-component secretion system. During this process, aggregation-prone, semi-folded curli subunits have to cross the periplasm and outer-membrane and self-assemble into surface-attached fibers. Two recent breakthroughs have provided molecular details regarding periplasmic chaperoning and subunit secretion. This review offers a combined perspective on these first mechanistic insights into the curli system.

  • JOURNAL ARTICLE
    Wilkinson RJ, Esmail H, Lesosky M, Lai RP, Wilkinson KA, Graham CM, Coussens AK, Oni T, Warwick J, Said-Hartley Q, Koegenelburg CF, Walzl G, Flynn JL, young DB, Barry CE, O'Garra Aet al.,

    [18F]-FDG PET/CT characterisation of progressive HIV-associated tuberculosis

    , Nature Medicine, ISSN: 1546-170X

    Tuberculosis is classically divided into states of latent infection and active disease. Usingcombined positron emission and computed tomography in 35 asymptomatic, antiretroviraltherapy naïve, HIV-1 infected adults with latent tuberculosis, we identified ten individualswith pulmonary abnormalities suggestive of subclinical, active disease who weresignificantly more likely to progress to clinical disease. Our findings challenge theconventional two-state paradigm and may aid future identification of biomarkers predictiveof progression.

  • JOURNAL ARTICLE
    Ale A, Crepin VF, Collins JW, Constantinou N, Habibzay M, Babtie AC, Frankel G, Stumpf MPHet al., 2017,

    Model of Host-Pathogen Interaction Dynamics Links In Vivo Optical Imaging and Immune Responses

    , INFECTION AND IMMUNITY, Vol: 85, ISSN: 0019-9567
  • JOURNAL ARTICLE
    Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux Aet al., 2017,

    RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa

    , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 114, Pages: 7707-7712, ISSN: 0027-8424
  • JOURNAL ARTICLE
    Bernal P, Allsopp LP, Filloux A, Llamas MAet al., 2017,

    The Pseudomonas putida T6SS is a plant warden against phytopathogens

    , ISME JOURNAL, Vol: 11, Pages: 972-987, ISSN: 1751-7362
  • JOURNAL ARTICLE
    Bosi E, Fondi M, Orlandini V, Perrin E, Maida I, de Pascale D, Tutino ML, Parrilli E, Lo Giudice A, Filloux A, Fani Ret al., 2017,

    The pangenome of (Antarctic) Pseudoalteromonas bacteria: evolutionary and functional insights

    , BMC GENOMICS, Vol: 18, ISSN: 1471-2164
  • JOURNAL ARTICLE
    Brown RL, Clarke TB, 2017,

    The regulation of host defences to infection by the microbiota

    , IMMUNOLOGY, Vol: 150, Pages: 1-6, ISSN: 0019-2805
  • JOURNAL ARTICLE
    Davies SK, Fearn S, Allsopp LP, Harrison F, Ware E, Diggle SP, Filloux A, McPhail DS, Bundy JGet al., 2017,

    Visualizing Antimicrobials in Bacterial Biofilms: Three-Dimensional Biochemical Imaging Using TOF-SIMS

    , MSPHERE, Vol: 2, ISSN: 2379-5042
  • JOURNAL ARTICLE
    Dominguez-Huettinger E, Boon NJ, Clarke TB, Tanaka RJet al., 2017,

    Mathematical Modeling of Streptococcus pneumoniae Colonization, Invasive Infection and Treatment

    , FRONTIERS IN PHYSIOLOGY, Vol: 8, ISSN: 1664-042X

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