Profiling of NMT activity and inhibition using Sortase-A

Congratulations to Andrea on the publication of her first first author paper!

Congratulations to third year PhD student Andrea Goya Grocin who has had her work published in Molecular and Cellular Proteomics.

The paper showcases a novel method for whole-proteome profiling of free N-terminal glycines through labeling with S. Aureus sortase A (SrtA) and its use for assessment of target engagement by an N-myristoyltransferase (NMT) inhibitor. The paper also reports SrtA substrate specificity in whole-cell lysates for the first time. The new methodology in this work is complementary to metabolic tagging strategies, and is the first approach for whole proteome gain-of signal readout for NMT inhibition in complex samples which are not suitable for metabolic tagging.

Previous group members Julia, Markus, Remi were also involved in this work, which was funded by CRUK and Horizon 2020.

Congratulations to all!