@article{Overby:2014:10.1073/pnas.1410602111,
author = {Overby, DR and Zhou, EH and Vargas-Pinto, R and Pedrigi, RM and Fuchshofer, R and Braakman, ST and Gupta, R and Perkumas, KM and Sherwood, JM and Vahabikashi, A and Quynh, D and Kim, JH and Ethier, CR and Stamer, WD and Fredberg, JJ and Johnson, M},
doi = {10.1073/pnas.1410602111},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
pages = {13876--13881},
title = {Altered mechanobiology of Schlemm's canal endothelial cells in glaucoma},
url = {http://dx.doi.org/10.1073/pnas.1410602111},
volume = {111},
year = {2014}
}

Download

TY  - JOUR
AB  - Increased flow resistance is responsible for the elevated intraocular pressure characteristic of glaucoma, but the cause of this resistance increase is not known. We tested the hypothesis that altered biomechanical behavior of Schlemm’s canal (SC) cells contributes to this dysfunction. We used atomic force microscopy, optical magnetic twisting cytometry, and a unique cell perfusion apparatus to examine cultured endothelial cells isolated from the inner wall of SC of healthy and glaucomatous human eyes. Here we establish the existence of a reduced tendency for pore formation in the glaucomatous SC cell—likely accounting for increased outflow resistance—that positively correlates with elevated subcortical cell stiffness, along with an enhanced sensitivity to the mechanical microenvironment including altered expression of several key genes, particularly connective tissue growth factor. Rather than being seen as a simple mechanical barrier to filtration, the endothelium of SC is seen instead as a dynamic material whose response to mechanical strain leads to pore formation and thereby modulates the resistance to aqueous humor outflow. In the glaucomatous eye, this process becomes impaired. Together, these observations support the idea of SC cell stiffness—and its biomechanical effects on pore formation—as a therapeutic target in glaucoma.
AU  - Overby,DR
AU  - Zhou,EH
AU  - Vargas-Pinto,R
AU  - Pedrigi,RM
AU  - Fuchshofer,R
AU  - Braakman,ST
AU  - Gupta,R
AU  - Perkumas,KM
AU  - Sherwood,JM
AU  - Vahabikashi,A
AU  - Quynh,D
AU  - Kim,JH
AU  - Ethier,CR
AU  - Stamer,WD
AU  - Fredberg,JJ
AU  - Johnson,M
DO  - 10.1073/pnas.1410602111
EP  - 13881
PY  - 2014///
SN  - 0027-8424
SP  - 13876
TI  - Altered mechanobiology of Schlemm's canal endothelial cells in glaucoma
T2  - Proceedings of the National Academy of Sciences of the United States of America
UR  - http://dx.doi.org/10.1073/pnas.1410602111
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000341988200053&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
VL  - 111
ER  - 

Download