Imperial College London
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DrAliceBanks

Faculty of Natural Sciences, Department of Life Sciences

Research Associate (Synthetic Biology)
 
 
 
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Contact

 

a.banks

 
 
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Location

 

Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Banks:2022:10.1016/j.csbj.2021.12.013,
author = {Banks, AM and Whitfield, CJ and Brown, SR and Fulton, DA and Goodchild, SA and Grant, C and Love, J and Lendrem, DW and Fieldsend, JE and Howard, TP},
doi = {10.1016/j.csbj.2021.12.013},
journal = {Computational and Structural Biotechnology Journal},
pages = {218--229},
title = {Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions},
url = {http://dx.doi.org/10.1016/j.csbj.2021.12.013},
volume = {20},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cell-free protein synthesis (CFPS) reactions have grown in popularity with particular interest in applications such as gene construct prototyping, biosensor technologies and the production of proteins with novel chemistry. Work has frequently focussed on optimising CFPS protocols for improving protein yield, reducing cost, or developing streamlined production protocols. Here we describe a statistical Design of Experiments analysis of 20 components of a popular CFPS reaction buffer. We simultaneously identify factors and factor interactions that impact on protein yield, rate of reaction, lag time and reaction longevity. This systematic experimental approach enables the creation of a statistical model capturing multiple behaviours of CFPS reactions in response to components and their interactions. We show that a novel reaction buffer outperforms the reference reaction by 400% and importantly reduces failures in CFPS across batches of cell lysates, strains of E. coli, and in the synthesis of different proteins. Detailed and quantitative understanding of how reaction components affect kinetic responses and robustness is imperative for future deployment of cell-free technologies.
AU  - Banks,AM
AU  - Whitfield,CJ
AU  - Brown,SR
AU  - Fulton,DA
AU  - Goodchild,SA
AU  - Grant,C
AU  - Love,J
AU  - Lendrem,DW
AU  - Fieldsend,JE
AU  - Howard,TP
DO  - 10.1016/j.csbj.2021.12.013
EP  - 229
PY  - 2022///
SN  - 2001-0370
SP  - 218
TI  - Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions
T2  - Computational and Structural Biotechnology Journal
UR  - http://dx.doi.org/10.1016/j.csbj.2021.12.013
UR  - https://www.sciencedirect.com/science/article/pii/S2001037021005213?via%3Dihub
UR  - http://hdl.handle.net/10044/1/93668
VL  - 20
ER  -
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