Imperial College London
Alternate

Professor Anil Anthony Bharath

Faculty of Engineering, Department of Bioengineering

Academic Director (Singapore)
 
 
 
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Contact

 

+44 (0)20 7594 5463a.bharath Website

 
 
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Location

 

4.12Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Othman:2016:10.1002/adhm.201501012,
author = {Othman, BA and Greenwood, C and Abuelela, AF and Bharath, AA and Chen, S and Theodorou, I and Douglas, T and Uchida, M and Ryan, M and Merzaban, JS and Porter, AE},
doi = {10.1002/adhm.201501012},
journal = {Advanced Healthcare Materials},
pages = {1310--1325},
title = {Correlative light-electron microscopy shows RGD-targeted ZnO nanoparticles dissolve in the intracellular environment of triple negative breast cancer cells and cause apoptosis with intra-tumor heterogeneity},
url = {http://dx.doi.org/10.1002/adhm.201501012},
volume = {5},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ZnO nanoparticles (NPs) are reported to show a high degree of cancer cell selectivity with potential use in cancer imaging and therapy. Questions remain about the mode by which the ZnO NPs cause cell death, whether they exert an intra- or extra-35 cellular effect, and the resistance among different cancer cell types to ZnO NP exposure. The present study quantified the variability between the cellular toxicity, dynamics of cellular uptake and dissolution of bare and RGD (Arg-Gly-Asp)-targeted ZnO NPs by MDA-MB-231 cells. Compared to bare ZnO NPs, RGD-targeting of the ZnO NPs to integrin αvβ3 receptors expressed on MDA-MB-231 cells appeared to increase the toxicity of the ZnO NPs to breast cancer cells at lower doses. Confocal microscopy of live MDA-MB-231 cells confirmed uptake of both classes of ZnO NPs with a commensurate rise in intracellular Zn2+ concentration prior to cell death. The response of the cells within the population to intracellular Zn2+ was highly heterogeneous. In addition, the results emphasize the utility of dynamic and quantitative imaging in understanding cell uptake and processing of targeted therapeutic ZnO NPs at the cellular level by heterogeneous cancer cell populations, which could be crucial for the development of optimized treatment strategies.
AU  - Othman,BA
AU  - Greenwood,C
AU  - Abuelela,AF
AU  - Bharath,AA
AU  - Chen,S
AU  - Theodorou,I
AU  - Douglas,T
AU  - Uchida,M
AU  - Ryan,M
AU  - Merzaban,JS
AU  - Porter,AE
DO  - 10.1002/adhm.201501012
EP  - 1325
PY  - 2016///
SN  - 2192-2640
SP  - 1310
TI  - Correlative light-electron microscopy shows RGD-targeted ZnO nanoparticles dissolve in the intracellular environment of triple negative breast cancer cells and cause apoptosis with intra-tumor heterogeneity
T2  - Advanced Healthcare Materials
UR  - http://dx.doi.org/10.1002/adhm.201501012
UR  - http://hdl.handle.net/10044/1/30266
VL  - 5
ER  -
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