Imperial College London

Professor Aldo R. Boccaccini

Faculty of EngineeringDepartment of Materials

Visiting Professor



+44 (0)20 7594 6731a.boccaccini




210Royal School of MinesSouth Kensington Campus






BibTex format

author = {Witt, R and Weigand, A and Boos, AM and Cai, A and Dippold, D and Boccaccini, AR and Schubert, DW and Hardt, M and Lange, C and Arkudas, A and Horch, RE and Beier, JP},
doi = {10.1186/s12860-017-0131-2},
journal = {BMC Cell Biology},
title = {Mesenchymal stem cells and myoblast differentiation under HGF and IGF-1 stimulation for 3D skeletal muscle tissue engineering},
url = {},
volume = {18},
year = {2017}

RIS format (EndNote, RefMan)

AB - BackgroundVolumetric muscle loss caused by trauma or after tumour surgery exceeds the natural regeneration capacity of skeletal muscle. Hence, the future goal of tissue engineering (TE) is the replacement and repair of lost muscle tissue by newly generating skeletal muscle combining different cell sources, such as myoblasts and mesenchymal stem cells (MSCs), within a three-dimensional matrix. Latest research showed that seeding skeletal muscle cells on aligned constructs enhance the formation of myotubes as well as cell alignment and may provide a further step towards the clinical application of engineered skeletal muscle.In this study the myogenic differentiation potential of MSCs upon co-cultivation with myoblasts and under stimulation with hepatocyte growth factor (HGF) and insulin-like growth factor-1 (IGF-1) was evaluated. We further analysed the behaviour of MSC-myoblast co-cultures in different 3D matrices.ResultsPrimary rat myoblasts and rat MSCs were mono- and co-cultivated for 2, 7 or 14 days. The effect of different concentrations of HGF and IGF-1 alone, as well as in combination, on myogenic differentiation was analysed using microscopy, multicolour flow cytometry and real-time PCR. Furthermore, the influence of different three-dimensional culture models, such as fibrin, fibrin-collagen-I gels and parallel aligned electrospun poly-ε-caprolacton collagen-I nanofibers, on myogenic differentiation was analysed. MSCs could be successfully differentiated into the myogenic lineage both in mono- and in co-cultures independent of HGF and IGF-1 stimulation by expressing desmin, myocyte enhancer factor 2, myosin heavy chain 2 and alpha-sarcomeric actinin. An increased expression of different myogenic key markers could be observed under HGF and IGF-1 stimulation. Even though, stimulation with HGF/IGF-1 does not seem essential for sufficient myogenic differentiation. Three-dimensional cultivation in fibrin-collagen-I gels induced higher levels of myogenic di
AU - Witt,R
AU - Weigand,A
AU - Boos,AM
AU - Cai,A
AU - Dippold,D
AU - Boccaccini,AR
AU - Schubert,DW
AU - Hardt,M
AU - Lange,C
AU - Arkudas,A
AU - Horch,RE
AU - Beier,JP
DO - 10.1186/s12860-017-0131-2
PY - 2017///
SN - 1471-2121
TI - Mesenchymal stem cells and myoblast differentiation under HGF and IGF-1 stimulation for 3D skeletal muscle tissue engineering
T2 - BMC Cell Biology
UR -
UR -
UR -
VL - 18
ER -