Imperial College London
Alternate

Professor Aldo R. Boccaccini

Faculty of Engineering, Department of Materials

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 6731a.boccaccini

 
 
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Location

 

210Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Qazi:2017:10.1002/jbm.a.36131,
author = {Qazi, TH and Hafeez, S and Schmidt, J and Duda, GN and Boccaccini, AR and Lippens, E},
doi = {10.1002/jbm.a.36131},
journal = {Journal of Biomedical Materials Research Part A},
pages = {2772--2782},
title = {Comparison of the effects of 45S5 and 1393 bioactive glass microparticles on hMSC behavior},
url = {http://dx.doi.org/10.1002/jbm.a.36131},
volume = {105},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bioactive glasses (BAGs) are highly interesting materials for bone regeneration applications in orthopedic and dental defects. It is quite well known that ionic release from BAGs influences cell behavior and function. Mindful of the clinical scenario, we hypothesized that local cell populations might additionally physically interact with the implanted BAG particles and respond differently than to just the ionic stimuli. We therefore studied the biological effect of two BAG types (45S5 and 1393) applied to human mesenchymal stromal cells (hMSCs) in three distinct presentation modes: (a) direct contact; and to dissolution products in (b) 2D, and (c) 3D culture. We furthermore investigated how the dose-dependence of these BAG particles, in concentrations ranging from 0.1 to 2.5 w/v %, influenced hMSC metabolic activity, proliferation, and cell spreading. These cellular functions were significantly hampered when hMSCs were exposed to high concentrations of either glasses, but the effects were more pronounced in the 45S5 groups and when the cells were in direct contact with the BAGs. Furthermore the biological effect of 1393 BAG outperformed that of 45S5 BAG in all tested presentation modes. These outcomes highlight the importance of investigating cell–BAG interactions in experimental set-ups that recapitulate host cell interactions with BAG particles.
AU  - Qazi,TH
AU  - Hafeez,S
AU  - Schmidt,J
AU  - Duda,GN
AU  - Boccaccini,AR
AU  - Lippens,E
DO  - 10.1002/jbm.a.36131
EP  - 2782
PY  - 2017///
SN  - 1549-3296
SP  - 2772
TI  - Comparison of the effects of 45S5 and 1393 bioactive glass microparticles on hMSC behavior
T2  - Journal of Biomedical Materials Research Part A
UR  - http://dx.doi.org/10.1002/jbm.a.36131
UR  - http://hdl.handle.net/10044/1/54062
VL  - 105
ER  -
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