Imperial College London

ProfessorAnneBowcock

Faculty of MedicineNational Heart & Lung Institute

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 1511a.bowcock

 
 
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Location

 

Guy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bowcock:1992,
author = {Bowcock, AM and Azuma, T and Barnes, RI and Wu, SH and Bell, GI and Taggart, RT},
journal = {Genomics},
pages = {398--402},
title = {Detection of a polymorphism within the pepsinogen C gene with PCR: construction of a linkage map around PGC from 6p11-6p21.3.},
url = {https://www.ncbi.nlm.nih.gov/pubmed/1427855},
volume = {14},
year = {1992}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - An insertion/deletion polymorphism between exons 7 and 8 of the pepsinogen C gene (PGC), previously detectable with Southern analysis, was formatted for detection with PCR. Alleles were rapidly typed by UV irradiation of ethidium bromide-stained agarose gels. Whereas Southern analysis revealed two alleles, the smaller fragments generated with PCR allowed the resolution of three alleles that were previously scored as a single allele and increased the heterozygosity of the system from 0.20 to 0.53. After a set of reference families was genotyped with the PCR-based polymorphism, a linkage map around the PGC gene on chromosome 6 was constructed. This included the HLA cluster and the highly informative D6S223 locus. PGC lies 22 cM proximal to HLA-DPB and between D6S5 and D6S4 at distances of 4.5 and 13.1 cM, respectively.
AU - Bowcock,AM
AU - Azuma,T
AU - Barnes,RI
AU - Wu,SH
AU - Bell,GI
AU - Taggart,RT
EP - 402
PY - 1992///
SN - 0888-7543
SP - 398
TI - Detection of a polymorphism within the pepsinogen C gene with PCR: construction of a linkage map around PGC from 6p11-6p21.3.
T2 - Genomics
UR - https://www.ncbi.nlm.nih.gov/pubmed/1427855
VL - 14
ER -