Imperial College London

ProfessorAnneBowcock

Faculty of MedicineNational Heart & Lung Institute

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 1511a.bowcock

 
 
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Location

 

Guy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bowcock:1989,
author = {Bowcock, AM and Ray, A and Erlich, H and Sehgal, PB},
journal = {Nucleic Acids Res},
pages = {6855--6864},
title = {Rapid detection and sequencing of alleles in the 3' flanking region of the interleukin-6 gene.},
url = {https://www.ncbi.nlm.nih.gov/pubmed/2789373},
volume = {17},
year = {1989}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The 3' flanking region of the interleukin 6 gene is polymorphic due to insertions of different size. Within this region lies a sequence of approximately 500 base pairs that is AT rich. Based on flanking sequence information we have constructed oligonucleotides which prime the polymerase chain reaction (PCR) and amplify this AT rich region. The amplification products visualized by agarose gel electrophoresis gave fragment sizes for both homozygous and heterozygous individuals that were concordant with those observed by conventional genomic blotting techniques. Alleles that could not be typed by Southern analysis were resolved with this approach. These results illustrate the value of PCR for the rapid detection of length polymorphisms such as those due to variable numbers of tandem repeats. In contrast to RFLP analysis this procedure takes less than a day to perform, is cheaper, avoids the use of radioactivity and requires far less substrate DNA. Three different human alleles were sequenced, and differences were detected that were due to both large duplications and loss of one or two bases, suggesting that AT rich regions identify highly polymorphic loci. The same primers also amplified non-human primate DNA, allowing a comparison of the human sequence with that of the common chimpanzee and baboon.
AU - Bowcock,AM
AU - Ray,A
AU - Erlich,H
AU - Sehgal,PB
EP - 6864
PY - 1989///
SN - 0305-1048
SP - 6855
TI - Rapid detection and sequencing of alleles in the 3' flanking region of the interleukin-6 gene.
T2 - Nucleic Acids Res
UR - https://www.ncbi.nlm.nih.gov/pubmed/2789373
VL - 17
ER -