Imperial College London
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DrAnneBurke-Gaffney

Faculty of Medicine, National Heart & Lung Institute

Lecturer
 
 
 
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Contact

 

a.burke-gaffney

 
 
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Location

 

GSB 311Royal BromptonRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Burke-Gaffney:1996:10.1152/ajpcell.1996.270.2.C552,
author = {Burke-Gaffney, A and Hellewell, PG},
doi = {10.1152/ajpcell.1996.270.2.C552},
journal = {Am J Physiol},
pages = {C552--C561},
title = {Regulation of ICAM-1 by dexamethasone in a human vascular endothelial cell line EAhy926.},
url = {http://dx.doi.org/10.1152/ajpcell.1996.270.2.C552},
volume = {270},
year = {1996}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Regulation by dexamethasone of intercellular adhesion molecule-1 (ICAM-1) in cultured monolayers of the human umbilical vein endothelial cell line EAhy926 was investigated. Tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) in combination or lipopolysaccharide (LPS) alone gave time- and dose-dependent increases in ICAM-1. Sustained expression of ICAM-1 was observed after short exposure (30 min) to TNF-alpha + IFN-gamma, but not to LPS. LPS-induced ICAM-1 expression was not inhibited by interleukin-1 (IL-1) receptor antagonist (0.01-100 micrograms/ml). Dexamethasone (1,000 nM) did not inhibit TNF-alpha + IFN-gamma-induced ICAM-1 expression or mRNA induction. In contrast, dexamethasone dose dependently (0.1-1,000 nM) inhibited LPS-induced ICAM-1 expression; however, its effect on mRNA was not established, because ICAM-1 mRNA induced by LPS was not detected at the time points investigated in this study (3 and 20 h). Adhesion of unstimulated human neutrophils to EAhy926 monolayers activated with TNF-alpha + IFN-gamma or LPS was increased in the presence of dexamethasone at low doses, whereas neutrophil adhesion to LPS- but not cytokine-stimulated endothelial cells was significantly reduced (P < 0.05) in the presence of a high dose of dexamethasone (1,000 nM). In conclusion, dexamethasone was demonstrated to regulate the expression and function of ICAM-1 in a stimulus-dependent manner.
AU  - Burke-Gaffney,A
AU  - Hellewell,PG
DO  - 10.1152/ajpcell.1996.270.2.C552
EP  - 561
PY  - 1996///
SN  - 0002-9513
SP  - 552
TI  - Regulation of ICAM-1 by dexamethasone in a human vascular endothelial cell line EAhy926.
T2  - Am J Physiol
UR  - http://dx.doi.org/10.1152/ajpcell.1996.270.2.C552
UR  - https://www.ncbi.nlm.nih.gov/pubmed/8779919
VL  - 270
ER  -
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