Imperial College London

Dr Abigail Clements

Faculty of Natural SciencesDepartment of Life Sciences

Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 7681a.clements

 
 
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Location

 

Lvl 1Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

22 results found

Berger CN, Crepin VF, Roumeliotis TI, Wright JC, Carson D, Pevsner-Fischer M, Furniss RCD, Dougan G, Bachash M, Yu L, Clements A, Collins JW, Elinav E, Larrouy-Maumus GJ, Choudhary JS, Frankel Get al., 2017, Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo., Cell Metab

The intestinal epithelial cells (IECs) that line the gut form a robust line of defense against ingested pathogens. We investigated the impact of infection with the enteric pathogen Citrobacter rodentium on mouse IEC metabolism using global proteomic and targeted metabolomics and lipidomics. The major signatures of the infection were upregulation of the sugar transporter Sglt4, aerobic glycolysis, and production of phosphocreatine, which mobilizes cytosolic energy. In contrast, biogenesis of mitochondrial cardiolipins, essential for ATP production, was inhibited, which coincided with increased levels of mucosal O2 and a reduction in colon-associated anaerobic commensals. In addition, IECs responded to infection by activating Srebp2 and the cholesterol biosynthetic pathway. Unexpectedly, infected IECs also upregulated the cholesterol efflux proteins AbcA1, AbcG8, and ApoA1, resulting in higher levels of fecal cholesterol and a bloom of Proteobacteria. These results suggest that C. rodentium manipulates host metabolism to evade innate immune responses and establish a favorable gut ecosystem.

JOURNAL ARTICLE

Furniss RCD, Clements A, 2017, Regulation of the Locus of Enterocyte Effacement in Attaching and Effacing Pathogens., J Bacteriol

Attaching and Effacing (AE) pathogens colonise the gut mucosa using a Type Three Secretion System (T3SS) and a suite of effector proteins. The Locus of Enterocyte Effacement (LEE) is the defining genetic feature of the AE pathogens, encoding the T3SS and the core effector proteins necessary for pathogenesis. Extensive research has revealed a complex regulatory network that senses and responds to a myriad of host and microbiota-derived signals in the infected gut to control transcription of the LEE. These signals include microbiota-liberated sugars and metabolites in the gut lumen, molecular oxygen at the gut epithelium and host hormones. Recent research has revealed that AE pathogens also perceive physical signals, such as attachment to the epithelium, and that the act of effector translocation remodels gene expression in infecting bacteria. In this review we summarise our knowledge to date and present an integrated view of how chemical, geographical and physical cues regulate the virulence program of AE pathogens during infection.

JOURNAL ARTICLE

Furniss RCD, Slater S, Frankel G, Clements Aet al., 2016, Enterohaemorrhagic E. coli modulates an ARF6:Rab35 signaling axis to prevent recycling endosome maturation during infection, JOURNAL OF MOLECULAR BIOLOGY, Vol: 428, Pages: 3399-3407, ISSN: 0022-2836

JOURNAL ARTICLE

Larrouy-Maumus G, Clements A, Filloux A, McCarthy RR, Mostowy Set al., 2016, Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry, JOURNAL OF MICROBIOLOGICAL METHODS, Vol: 120, Pages: 68-71, ISSN: 0167-7012

JOURNAL ARTICLE

Mavridou DAI, Gonzalez D, Clements A, Foster KRet al., 2016, The pUltra plasmid series: A robust and flexible tool for fluorescent labeling of Enterobacteria, PLASMID, Vol: 87-88, Pages: 65-71, ISSN: 0147-619X

JOURNAL ARTICLE

Clements A, Stoneham CA, Furniss RCD, Frankel Get al., 2014, Enterohaemorrhagic Escherichia coli inhibits recycling endosome function and trafficking of surface receptors, CELLULAR MICROBIOLOGY, Vol: 16, Pages: 1693-1705, ISSN: 1462-5814

JOURNAL ARTICLE

Young JC, Clements A, Lang AE, Garnett JA, Munera D, Arbeloa A, Pearson J, Hartland EL, Matthews SJ, Mousnier A, Barry DJ, Way M, Schlosser A, Aktories K, Frankel Get al., 2014, The Escherichia coli effector EspJ blocks Src kinase activity via amidation and ADP ribosylation, NATURE COMMUNICATIONS, Vol: 5, ISSN: 2041-1723

JOURNAL ARTICLE

Clements A, Berger CN, Lomma M, Frankel Get al., 2013, Type 3 secretion effectors, Escherichia coliPathotypes and Principles of Pathogenesis, Editors: Donnenberg, ISBN: 978-0-12-397048-0

BOOK CHAPTER

Raymond B, Young JC, Pallett M, Endres RG, Clements A, Frankel Get al., 2013, Subversion of trafficking, apoptosis, and innate immunity by type III secretion system effectors, TRENDS IN MICROBIOLOGY, Vol: 21, Pages: 430-439, ISSN: 0966-842X

JOURNAL ARTICLE

Velkov T, Soon RL, Chong PL, Huang JX, Cooper MA, Azad MAK, Baker MA, Thompson PE, Roberts K, Nation RL, Clements A, Strugnell RA, Li Jet al., 2013, Molecular basis for the increased polymyxin susceptibility of Klebsiella pneumoniae strains with under-acylated lipid A, INNATE IMMUNITY, Vol: 19, Pages: 265-277, ISSN: 1753-4259

JOURNAL ARTICLE

Clements A, Young JC, Constantinou N, Frankel Get al., 2012, Infection strategies of enteric pathogenic Escherichia coli., Gut Microbes, Vol: 3, Pages: 71-87

Enteric Escherichia coli (E. coli) are both natural flora of humans and important pathogens causing significant morbidity and mortality worldwide. Traditionally enteric E. coli have been divided into 6 pathotypes, with further pathotypes often proposed. In this review we suggest expansion of the enteric E. coli into 8 pathotypes to include the emerging pathotypes of adherent invasive E. coli (AIEC) and Shiga-toxin producing enteroaggregative E. coli (STEAEC). The molecular mechanisms that allow enteric E. coli to colonize and cause disease in the human host are examined and for two of the pathotypes that express a type 3 secretion system (T3SS) we discuss the complex interplay between translocated effectors and manipulation of host cell signaling pathways that occurs during infection.

JOURNAL ARTICLE

Wong ARC, Clements A, Raymond B, Crepin VF, Frankel Get al., 2012, The Interplay between the Escherichia coli Rho Guanine Nucleotide Exchange Factor Effectors and the Mammalian RhoGEF Inhibitor EspH, MBIO, Vol: 3, ISSN: 2150-7511

JOURNAL ARTICLE

Clements A, Smollett K, Lee SF, Hartland EL, Lowe M, Frankel Get al., 2011, EspG of enteropathogenic and enterohemorrhagic E. coli binds the Golgi matrix protein GM130 and disrupts the Golgi structure and function, CELLULAR MICROBIOLOGY, Vol: 13, Pages: 1429-1439, ISSN: 1462-5814

JOURNAL ARTICLE

Robinson KS, Clements A, Williams AC, Berger CN, Frankel Get al., 2011, Bax Inhibitor 1 in apoptosis and disease, ONCOGENE, Vol: 30, Pages: 2391-2400, ISSN: 0950-9232

JOURNAL ARTICLE

Wilksch JJ, Yang J, Clements A, Gabbe JL, Short KR, Cao H, Cavaliere R, James CE, Whitchurch CB, Schembri MA, Chuah MLC, Liang Z-X, Wijburg OL, Jenney AW, Lithgow T, Strugnell RAet al., 2011, MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression, PLOS PATHOGENS, Vol: 7, ISSN: 1553-7366

JOURNAL ARTICLE

Alcock F, Clements A, Webb C, Lithgow Tet al., 2010, Tinkering Inside the Organelle, SCIENCE, Vol: 327, Pages: 649-650, ISSN: 0036-8075

JOURNAL ARTICLE

Clements A, Bursac D, Gatsos X, Perry AJ, Civciristov S, Celik N, Likic VA, Poggio S, Jacobs-Wagner C, Strugnell RA, Lithgow Tet al., 2009, The reducible complexity of a mitochondrial molecular machine, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 106, Pages: 15791-15795, ISSN: 0027-8424

JOURNAL ARTICLE

Clements A, Gaboriaud F, Duval JFL, Farn JL, Jenney AW, Lithgow T, Wijburg OLC, Hartland EL, Strugnell RAet al., 2008, The Major Surface-Associated Saccharides of Klebsiella pneumoniae Contribute to Host Cell Association, PLOS ONE, Vol: 3, ISSN: 1932-6203

JOURNAL ARTICLE

Clements A, Jenney AW, Farn JL, Brown LE, Deliyannis G, Hartland EL, Pearse MJ, Maloney MB, Wesselingh SL, Wijburg OL, Strugnell RAet al., 2008, Targeting subcapsular antigens for prevention of Klebsiella pneumoniae infections, VACCINE, Vol: 26, Pages: 5649-5653, ISSN: 0264-410X

JOURNAL ARTICLE

Clements A, Tull D, Jenney AW, Farn JL, Kim S-H, Bishop RE, McPhee JB, Hancock REW, Hartland EL, Pearse MJ, Wijburg OLC, Jackson DC, McConville MJ, Strugnell RAet al., 2007, Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 282, Pages: 15569-15577, ISSN: 0021-9258

JOURNAL ARTICLE

Carter DM, Miousse IR, Gagnon J-N, Martinez É, Clements A, Lee J, Hancock MA, Gagnon H, Pawelek PD, Coulton JWet al., 2006, Interactions between TonB from Escherichia coli and the Periplasmic Protein FhuD, Journal of Biological Chemistry, Vol: 281, Pages: 35413-35424, ISSN: 0021-9258

JOURNAL ARTICLE

Jenney AW, Clements A, Farn JL, Wijburg OL, McGlinchey A, Spelman DW, Pitt TL, Kaufmann ME, Liolios L, Moloney MB, Wesselingh SL, Strugnell RAet al., 2006, Seroepidemiology of Klebsiella pneumoniae in an Australian tertiary hospital and its implications for vaccine development, JOURNAL OF CLINICAL MICROBIOLOGY, Vol: 44, Pages: 102-107, ISSN: 0095-1137

JOURNAL ARTICLE

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