Imperial College London
Alternate

Professor the Lord Darzi of Denham PC KBE FRS FMedSci HonFREng

Faculty of Medicine, Department of Surgery & Cancer

Co-Director of the IGHI, Professor of Surgery
 
 
 
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Contact

 

+44 (0)20 3312 1310a.darzi

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Harling:2016:ejcts/ezw245,
author = {Harling, L and Lambert, J and Ashrafian, H and Darzi, A and Gooderham, N and Athanasiou, T},
doi = {ejcts/ezw245},
journal = {European Journal of Cardio-thoracic Surgery},
pages = {73--78},
title = {Elevated serum microRNA 483-5p levels may predict patients at risk of post-operative atrial fibrillation},
url = {http://dx.doi.org/10.1093/ejcts/ezw245},
volume = {51},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Objective: Atrial fibrillation (POAF) is the commonest post-operative cardiac arrhythmia, affecting approximately 1 in 3 patients undergoing coronary artery bypass grafting (CABG). Although its aetiology is complex, atrial substrate changes may pre-dispose to its onset. This study aims to ascertain the atrial microRNA signature of POAF and determine the potential for circulating microRNA as a pre-operative biomarker for this arrhythmia.Methods: 34 patients undergoing non-emergent, on-pump CABG were prospectively recruited. Right atrial biopsies were taken intra-operatively and snap frozen for RNA extraction. Plasma was obtained at 24 hours pre-operatively and at 2 and 4-days post-operatively. POAF was defined by continuous Holter recording. Inter-group comparisons were performed using student t-test or ANOVA as required. Receiver operating characteristics (ROC) analysis was used to determine the diagnostic accuracy of pre-operative serum miRNA as a POAF biomarker. Results: 16 microRNA were differentially expressed in the atrial myocardium of POAF patients when compared to those maintaining sinus rhythm (SR). MiR-208a was the most under-expressed (FC:2.458) and miR-483-5p the most over-expressed (FC:1.804). Mir-483-5p also demonstrated significant overexpression in the pre-operative serum of these patients, with ROC analysis demonstrating an overall predictive accuracy of 78%.  Conclusions: This study provides the first description of atrial myocardial and circulating plasma microRNA in POAF patients. Our findings suggest POAF may be associated with pre-existing atrial substrate differences predisposing to arrhythmogenesis. Moreover, this study highlights the potential for miR-483-5p in biomarker development. Further work must now perform prospective, targeted validation of these results in a larger patient cohort.
AU  - Harling,L
AU  - Lambert,J
AU  - Ashrafian,H
AU  - Darzi,A
AU  - Gooderham,N
AU  - Athanasiou,T
DO  - ejcts/ezw245
EP  - 78
PY  - 2016///
SN  - 1873-734X
SP  - 73
TI  - Elevated serum microRNA 483-5p levels may predict patients at risk of post-operative atrial fibrillation
T2  - European Journal of Cardio-thoracic Surgery
UR  - http://dx.doi.org/10.1093/ejcts/ezw245
UR  - http://hdl.handle.net/10044/1/34349
VL  - 51
ER  -
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