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BibTex format

@article{De:2016:10.1038/srep23063,
author = {De, Simone A and Sanz-Hernandez, M and Vostrikov, V and Veglia, G},
doi = {10.1038/srep23063},
journal = {Scientific Reports},
title = {Accurate Determination of Conformational Transitions in Oligomeric Membrane Proteins},
url = {http://dx.doi.org/10.1038/srep23063},
volume = {6},
year = {2016}
}

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TY  - JOUR
AB  - Thestructuraldynamicsgoverningcollectivemotionsinoligomericmembraneproteinsplaykeyrolesinvital biomolecularprocesses atcellularmembranes.Inthisstudy,wepresentastructuralrefinementapproachthatcombinessolid-stateNMRexperimentsandmolecularsimulations toaccuratelydescribeconcertedconformationaltransitions identifying theoverallstructural,dynamical, andtopologicalstatesofoligomericmembraneproteins.Theaccuracyofthestructuralensemblesgeneratedwiththismethodisshowntoreach thestatisticalerrorlimit,andis furtherdemonstratedbycorrectlyreproducing orthogonalNMRdata. Wedemonstratetheaccuracy ofthis approachby characterisingthepentameric stateofphospholamban,akeyplayerintheregulationofcalciumuptakeinthesarcoplasmicreticulum,andbyprobingits dynamicalactivationuponphosphorylation.Ourresultsunderlinetheimportanceofusinganensembleapproachtocharacterise theconformationaltransitionsthatareoftenresponsibleforthebiologicalfunction of oligomericmembraneproteinstates.
AU  - De,Simone A
AU  - Sanz-Hernandez,M
AU  - Vostrikov,V
AU  - Veglia,G
DO  - 10.1038/srep23063
PY  - 2016///
SN  - 2045-2322
TI  - Accurate Determination of Conformational Transitions in Oligomeric Membrane Proteins
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep23063
UR  - http://hdl.handle.net/10044/1/29908
VL  - 6
ER  -

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ProfessorAlfonsoDe Simone

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