Imperial College London

DrArmandoDel Rio Hernandez

Faculty of EngineeringDepartment of Bioengineering

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5187a.del-rio-hernandez

 
 
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Location

 

308Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Robinson:2016:10.1242/bio.017632,
author = {Robinson, BK and Cortes, E and Rice, AJ and Sarper, M and del, Rio Hernandez A},
doi = {10.1242/bio.017632},
journal = {Biology Open},
pages = {875--882},
title = {Quantitative analysis of 3D extracellular matrix remodelling bypancreatic stellate cells},
url = {http://dx.doi.org/10.1242/bio.017632},
volume = {5},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Extracellular matrix (ECM) remodelling is integral to numerousphysiological and pathological processes in biology, such asembryogenesis, wound healing, fibrosis and cancer. Until recently,most cellular studies have been conducted on 2D environmentswhere mechanical cues significantly differ from physiologicallyrelevant 3D environments, impacting cellular behaviour andmasking the interpretation of cellular function in health and disease.We present an integrated methodology where cell-ECM interactionscan be investigated in 3D environments via ECM remodelling.Monitoring and quantification of collagen-I structure in remodelledmatrices, through designated algorithms, show that 3D matrices canbe used to correlate remodelling with increased ECM stiffnessobserved in fibrosis. Pancreatic stellate cells (PSCs) are the keyeffectors of the stromal fibrosis associated to pancreatic cancer. Weuse PSCs to implement our methodology and demonstrate that PSCmatrix remodelling capabilities depend on their contractile machineryand β1 integrin-mediated cell-ECM attachment.
AU - Robinson,BK
AU - Cortes,E
AU - Rice,AJ
AU - Sarper,M
AU - del,Rio Hernandez A
DO - 10.1242/bio.017632
EP - 882
PY - 2016///
SN - 2046-6390
SP - 875
TI - Quantitative analysis of 3D extracellular matrix remodelling bypancreatic stellate cells
T2 - Biology Open
UR - http://dx.doi.org/10.1242/bio.017632
UR - http://hdl.handle.net/10044/1/31956
VL - 5
ER -