Imperial College London

DrArmandoDel Rio Hernandez

Faculty of EngineeringDepartment of Bioengineering

Senior Lecturer
 
 
 
//

Contact

 

+44 (0)20 7594 5187a.del-rio-hernandez

 
 
//

Location

 

308Bessemer BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Haining:2016:10.1021/acsnano.6b01658,
author = {Haining, AW and von, Essen M and Attwood, SJ and Hytonen, VP and del, Rio Hernandez A},
doi = {10.1021/acsnano.6b01658},
journal = {ACS Nano},
pages = {6648--6658},
title = {All Subdomains of the Talin Rod Are Mechanically Vulnerable and May Contribute to Cellular Mechanosensing},
url = {http://dx.doi.org/10.1021/acsnano.6b01658},
volume = {10},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Although the relevance of mechanotransduction in cell signalling is currently appreciated, themechanisms that drive this process remain largely unknown. Mechanical unfolding of proteins maytrigger distinct downstream signals in cells, providing a mechanism for cellular mechanotransduction.Force-induced unfolding of talin, a prominent focal adhesion protein, has been demonstrated previouslyfor a small portion of its rod domain. Here, using single-molecule atomic force microscopy (smAFM), weshow that the entire talin rod can be unfolded by mechanical extension, over a physiological range offorces between 10 – 40 pN. We also demonstrate, through a combination of smAFM and steeredmolecular dynamics (SMD), that the different bundles within the talin rod exhibit a distinct hierarchy ofmechanical stability. These results provide a mechanism by which different force conditions within thecell control a graduated unfolding of the talin rod. Mechanical unfolding of the rod subdomains, and thesubsequent effect on talin’s binding interactions, would allow for a finely-tuned cellular response tointernally or externally applied forces.
AU - Haining,AW
AU - von,Essen M
AU - Attwood,SJ
AU - Hytonen,VP
AU - del,Rio Hernandez A
DO - 10.1021/acsnano.6b01658
EP - 6658
PY - 2016///
SN - 1936-086X
SP - 6648
TI - All Subdomains of the Talin Rod Are Mechanically Vulnerable and May Contribute to Cellular Mechanosensing
T2 - ACS Nano
UR - http://dx.doi.org/10.1021/acsnano.6b01658
UR - http://hdl.handle.net/10044/1/34462
VL - 10
ER -