Imperial College London

DrArmandoDel Rio Hernandez

Faculty of EngineeringDepartment of Bioengineering

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5187a.del-rio-hernandez

 
 
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Location

 

308Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Attwood:2016:10.1038/srep34334,
author = {Attwood, S and Cortes, E and Haining and robinson, B and Li, D and Gautrot, J and Del, Rio Hernandez AE},
doi = {10.1038/srep34334},
journal = {Scientific Reports},
title = {Adhesive ligand tether length affects the size and length of focal adhesions and influences cell spreading and attachment},
url = {http://dx.doi.org/10.1038/srep34334},
volume = {6},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Cells are known to respond to physical cues from their microenvironment such as matrix rigidity.Discrete adhesive ligands within flexible strands of fibronectin connect cell surface integrins to thebroader extracellular matrix and are thought to mediate mechanosensing through the cytoskeletonintegrin-ECMlinkage. We set out to determine if adhesive ligand tether length is another physical cuethat cells can sense. Substrates were covalently modified with adhesive arginylglycylaspartic acid (RGD)ligands coupled with short (9.5nm), medium (38.2nm) and long (318nm) length inert polyethyleneglycol tethers. The size and length of focal adhesions of human foreskin fibroblasts gradually decreasedfrom short to long tethers. Furthermore, we found cell adhesion varies in a linker length dependentmanner with a remarkable 75% reduction in the density of cells on the surface and a 50% reduction incell area between the shortest and longest linkers. We also report the interplay between RGD ligandconcentration and tether length in determining cellular spread area. Our findings show that withoutvarying substrate rigidity or ligand density, tether length alone can modulate cellular behaviour.
AU - Attwood,S
AU - Cortes,E
AU - Haining
AU - robinson,B
AU - Li,D
AU - Gautrot,J
AU - Del,Rio Hernandez AE
DO - 10.1038/srep34334
PY - 2016///
SN - 2045-2322
TI - Adhesive ligand tether length affects the size and length of focal adhesions and influences cell spreading and attachment
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/srep34334
UR - http://hdl.handle.net/10044/1/40336
VL - 6
ER -