Imperial College London
Alternate

DrArmandoDel Rio Hernandez

Faculty of Engineering, Department of Bioengineering

Reader in Cellular and Molecular Mechanotransduction
 
 
 
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Contact

 

+44 (0)20 7594 5187a.del-rio-hernandez

 
 
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Location

 

308Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Haining:2016:10.1021/acsnano.6b01658,
author = {Haining, AW and von, Essen M and Attwood, SJ and Hytonen, VP and del, Rio Hernandez A},
doi = {10.1021/acsnano.6b01658},
journal = {ACS Nano},
pages = {6648--6658},
title = {All subdomains of the talin rod are mechanically vulnerable and may contribute to cellular mechanosensing},
url = {http://dx.doi.org/10.1021/acsnano.6b01658},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Although the relevance of mechanotransduction in cell signaling is currently appreciated, the mechanisms that drive this process remain largely unknown. Mechanical unfolding of proteins may trigger distinct downstream signals in cells, providing a mechanism for cellular mechanotransduction. Force-induced unfolding of talin, a prominent focal adhesion protein, has been demonstrated previously for a small portion of its rod domain. Here, using single-molecule atomic force microscopy (smAFM), we show that the entire talin rod can be unfolded by mechanical extension, over a physiological range of forces between 10 and 40 pN. We also demonstrate, through a combination of smAFM and steered molecular dynamics, that the different bundles within the talin rod exhibit a distinct hierarchy of mechanical stability. These results provide a mechanism by which different force conditions within the cell control a graduated unfolding of the talin rod. Mechanical unfolding of the rod subdomains, and the subsequent effect on talin’s binding interactions, would allow for a finely tuned cellular response to internally or externally applied forces.
AU  - Haining,AW
AU  - von,Essen M
AU  - Attwood,SJ
AU  - Hytonen,VP
AU  - del,Rio Hernandez A
DO  - 10.1021/acsnano.6b01658
EP  - 6658
PY  - 2016///
SN  - 1936-0851
SP  - 6648
TI  - All subdomains of the talin rod are mechanically vulnerable and may contribute to cellular mechanosensing
T2  - ACS Nano
UR  - http://dx.doi.org/10.1021/acsnano.6b01658
UR  - https://pubs.acs.org/doi/10.1021/acsnano.6b01658
UR  - http://hdl.handle.net/10044/1/34462
VL  - 10
ER  -
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