Imperial College London
Alternate

DrArmandoDel Rio Hernandez

Faculty of Engineering, Department of Bioengineering

Reader in Cellular and Molecular Mechanotransduction
 
 
 
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Contact

 

+44 (0)20 7594 5187a.del-rio-hernandez

 
 
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Location

 

308Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kis:2016:10.1038/srep29643,
author = {Kis, Z and Rodin, T and Zafar, A and Lai, Z and Freke, G and Fleck, O and Del, Rio Hernandez A and Towhidi, L and Pedrigi, R and Homma, T and Krams, R},
doi = {10.1038/srep29643},
journal = {Scientific Reports},
title = {Development of a synthetic gene network to module gene expression by mechanical forces},
url = {http://dx.doi.org/10.1038/srep29643},
volume = {6},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The majority of (mammalian) cells in our body are sensitive to mechanical forces, but little work hasbeen done to develop assays to monitor mechanosensor activity. Furthermore, it is currently impossibleto use mechanosensor activity to drive gene expression. To address these needs, we developed thefrst mammalian mechanosensitive synthetic gene network to monitor endothelial cell shear stresslevels and directly modulate expression of an atheroprotective transcription factor by shear stress. Thetechnique is highly modular, easily scalable and allows graded control of gene expression by mechanicalstimuli in hard-to-transfect mammalian cells. We call this new approach mechanosyngenetics. To insertthe gene network into a high proportion of cells, a hybrid transfection procedure was developed thatinvolves electroporation, plasmids replication in mammalian cells, mammalian antibiotic selection,a second electroporation and gene network activation. This procedure takes 1 week and yielded over60% of cells with a functional gene network. To test gene network functionality, we developed a fowsetup that exposes cells to linearly increasing shear stress along the length of the fow channel foor.Activation of the gene network varied logarithmically as a function of shear stress magnitude.
AU  - Kis,Z
AU  - Rodin,T
AU  - Zafar,A
AU  - Lai,Z
AU  - Freke,G
AU  - Fleck,O
AU  - Del,Rio Hernandez A
AU  - Towhidi,L
AU  - Pedrigi,R
AU  - Homma,T
AU  - Krams,R
DO  - 10.1038/srep29643
PY  - 2016///
SN  - 2045-2322
TI  - Development of a synthetic gene network to module gene expression by mechanical forces
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep29643
UR  - http://hdl.handle.net/10044/1/34697
VL  - 6
ER  -
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