Publications
215 results found
Crisanti A, 2006, Unravelling how the immune systems 'sees' the antigen repertoire of <i>Plasmodium falciparum</i>, PARASITE IMMUNOLOGY, Vol: 28, Pages: 249-250, ISSN: 0141-9838
Catteruccia F, Benton JP, Crisanti A, 2005, An <i>Anopheles</i> transgenic sexing strain for vector control, NATURE BIOTECHNOLOGY, Vol: 23, Pages: 1414-1417, ISSN: 1087-0156
- Author Web Link
- Cite
- Citations: 158
Scali C, Catteruccia F, Li QX, et al., 2005, Identification of sex-specific transcripts of the <i>Anopheles gambiae</i> <i>doublesex</i> gene, JOURNAL OF EXPERIMENTAL BIOLOGY, Vol: 208, Pages: 3701-3709, ISSN: 0022-0949
- Author Web Link
- Cite
- Citations: 88
Scali C, Catteruccia F, Li Q, et al., 2005, Identification of sex-specific transcripts of the <i>Anopheles gambiae</i> doublesex gene (vol 208, pg 3701, 2005), JOURNAL OF EXPERIMENTAL BIOLOGY, Vol: 208, Pages: 3999-3999, ISSN: 0022-0949
Tewari R, Rathore D, Crisanti A, 2005, Motility and infectivity of <i>Plasmodium berghei</i> sporozoites expressing avian <i>Plasmodium gallinaceum</i> circumsporozoite protein, CELLULAR MICROBIOLOGY, Vol: 7, Pages: 699-707, ISSN: 1462-5814
- Author Web Link
- Cite
- Citations: 20
Young K, Frodsham A, Doumbo OK, et al., 2005, Inverse associations of human leukocyte antigen and malaria parasite types in two West African populations, INFECTION AND IMMUNITY, Vol: 73, Pages: 953-955, ISSN: 0019-9567
- Author Web Link
- Cite
- Citations: 9
Tewari R, Ogun SA, Gunaratne RS, et al., 2005, Disruption of <i>Plasmodium berghei</i> merozoite surface protein 7 gene modulates parasite growth in vivo, BLOOD, Vol: 105, Pages: 394-396, ISSN: 0006-4971
- Author Web Link
- Cite
- Citations: 28
Bacarese-Hamilton T, Gray J, Ardizzoni A, et al., 2005, Allergen microarrays., Methods Mol Med, Vol: 114, Pages: 195-207, ISSN: 1543-1894
Allergy affects more than 25% of Western populations (1) and is estimated to be the sixth leading cause of chronic disease in the United States and Western Europe. The complexity of the condition is such that hundreds of common allergens have been described, and in order to maximize diagnostic efficiency there is an urgent clinical requirement for assays to provide multiple-allergen determination in a timely and cost-effective manner. Miniaturized immunoassays that utilize protein microarray technology now offer the possibility of circumventing most of the current limitations in the serodiagnosis of allergic disease. The heterogeneous nature of allergens presents many challenges in all aspects of developing such arrays, from immobilization of the capture molecule to detection of the bound ligand. In addition, there is no simple method of protein amplification (such as PCR for nucleic acids), and stabilization is yet a further major consideration. Notwithstanding these challenges, protein microarrays have been developed for the serodiagnosis of allergies and other complex clinical conditions. These assays exhibit good analytical and clinical performance and deliver significant advantages in convenience and cost compared with traditional ELISA test formats. This chapter details the techniques employed in the construction and processing of an allergen array specific for the serodiagnosis of allergic disease. An overview of protein microarray technology is provided and the principles that underpin the suitability for use of this technology in the identification and measurement of particular proteins in patient sera (serum profiling) are discussed.
Rao F, Crisanti A, Ritort F, 2004, Frequency-domain study of α relaxation in the random orthogonal model, 9th International Workshop on Disordered Systems, Publisher: TAYLOR & FRANCIS LTD, Pages: 1389-1395, ISSN: 1478-6435
Okhuysen PC, Rogers GA, Crisanti A, et al., 2004, Antibody response of healthy adults to recombinant thrombospondin-related adhesive protein of <i>Cryptosporidium</i> 1 after experimental exposure to <i>Cryptosporidium</i> oocysts, CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, Vol: 11, Pages: 235-238, ISSN: 1071-412X
- Author Web Link
- Cite
- Citations: 10
Bacarese-Hamilton T, Ardizzoni A, Gray J, et al., 2004, Protein arrays for serodiagnosis of disease., Methods Mol Biol, Vol: 264, Pages: 271-283, ISSN: 1064-3745
Protein microarrays offer the possibility to circumvent most of the current limitations in the serodiagnosis of allergy, autoimmune, and infectious disease by allowing the simultaneous, multiparametric determination of specific subclasses of antibodies directed against many pathogenic antigens. Microarray immunoassays have been developed with these characteristics. A first-generation assay, for the serodiagnosis of infectious disease, allows the determination of IgG and IgM antibodies to various viral and bacterial antigens. In addition, a second-generation assay, designed for the serodiagnosis of allergic disease, permits the determination of IgE antibodies to various allergens implicated in allergic disease. Slides printed with antibody dilution curves and antigen are first incubated with serum samples and then subsequently with secondary antibodies. For detection of human IgG and IgM, fluorescently labeled secondary antibodies are employed. However, because of low-level concentrations of circulating IgE antibodies, a more sensitive protocol is required for human IgE detection. Here, fluorescence is delivered via the coupling of the secondary antibody to tyramide signal-amplification reagentry. Human IgG, IgM, or IgE bound to the printed antigens can then be revealed by confocal scanning microscopy and quantified with internal calibration curves. Generation of analytical and clinical data have demonstrated that the microarray test format provides equivalent performance to enzyme-linked immunosorbent assay (ELISA) tests and offers a significant advantage in convenience and cost when compared to traditional test formats.
Bacarese-Hamilton T, Mezzasoma L, Ardizzoni A, et al., 2004, Serodiagnosis of infectious diseases with antigen microarrays, JOURNAL OF APPLIED MICROBIOLOGY, Vol: 96, Pages: 10-17, ISSN: 1364-5072
- Author Web Link
- Cite
- Citations: 59
Mortlock A, Low W, Crisanti A, 2003, Suppression of gene expression by a cell-permeable Tet repressor, NUCLEIC ACIDS RESEARCH, Vol: 31, ISSN: 0305-1048
- Author Web Link
- Cite
- Citations: 7
Puebla I, Esseghir S, Mortlock A, et al., 2003, A recombinant H1 histone-based system for efficient delivery of nucleic acids, JOURNAL OF BIOTECHNOLOGY, Vol: 105, Pages: 215-226, ISSN: 0168-1656
- Author Web Link
- Cite
- Citations: 39
Dolo A, Poudiougo B, Modiano D, et al., 2003, [Epidemiology of malaria in a village of Sudanese savannah in Mali (Bancoumana). Anti-TRAP and anti-CS humoral immunity response]., Bull Soc Pathol Exot, Vol: 96, Pages: 287-290, ISSN: 0037-9085
Vaccine development research is an important component of malaria control strategies. Thrombospondin related anonymous protein (TRAP) and the circumsporozoite (CS) protein are two antigens of sporozoite surface. Immune response to these two antigens may contribute to the development of anti-sporozoite vaccine. Recent studies suggest that antibodies anti-TRAP may partially block sporozoites penetration in hepatocyte, and thereby reducing malaria morbidity. We carried out a study to assess the seroprevalence of anti-TRAP and anti-CS antibodies and to identify a possible role of these antibodies on malaria morbidity in children 1-9 years old living in a rural hyperendemic village. We performed 5 cross sectional surveys and a longitudinal follow up in 1993 and 1994. During each cross sectional study, children were examined for fever and splenomegaly; all febrile children received thick film examination, and serologic analysis was performed in one third of these, randomly selected. The results show that the seroprevalence of anti-TRAP and anti-CS varied with age and season (p < 0.05). Association between the prevalence of anti-TRAP and splenomegaly was observed during two cross sectional surveys (June and October 1993). The presence of anti-TRAP antibody was associated with Plasmodium falciparum infection at the beginning of the transmission season (June 1993 and July 1994). A negative association between the level of anti-TRAP title and parasitemia was observed (March and October 1994). These findings suggest no clear evidence of the protective role of anti-TRAP antibodies in uncomplicated malaria, possibly due to the limited persistence of these antibodies under natural situations.
Brown AE, Bugeon L, Crisanti A, et al., 2003, Stable and heritable gene silencing in the malaria vector <i>Anopheles stephensi</i> -: art. no. e85, NUCLEIC ACIDS RESEARCH, Vol: 31, ISSN: 0305-1048
- Author Web Link
- Cite
- Citations: 46
Bacarese-Hamilton T, Gray J, Crisanti A, 2003, Protein microarray technology for unraveling the antibody specificity repertoire against microbial proteomes, CURRENT OPINION IN MOLECULAR THERAPEUTICS, Vol: 5, Pages: 278-284, ISSN: 1464-8431
- Author Web Link
- Cite
- Citations: 14
Bacarese-Hamilton T, Ardizzoni A, Gray J, et al., 2003, High performance microarray immunoassays for serodiagnosis of infectious disease and allergies., 55th Annual Meeting of the American-Association-for-Clinical-Chemiatry, Publisher: AMER ASSOC CLINICAL CHEMISTRY, Pages: A13-A13, ISSN: 0009-9147
Brown AE, Crisanti A, Catteruccia F, 2003, Comparative analysis of DNA vectors at mediating RNAi in <i>Anopheles</i> mosquito cells and larvae, JOURNAL OF EXPERIMENTAL BIOLOGY, Vol: 206, Pages: 1817-1823, ISSN: 0022-0949
- Author Web Link
- Cite
- Citations: 16
Catteruccia F, Godfray HCJ, Crisanti A, 2003, Impact of genetic manipulation on the fitness of <i>Anopheles stephensi</i> mosquitoes, SCIENCE, Vol: 299, Pages: 1225-1227, ISSN: 0036-8075
- Author Web Link
- Cite
- Citations: 156
Tewari R, Spaccapelo R, Bistoni F, et al., 2002, Function of region I and II adhesive motifs of <i>Plasmodium falciparum</i> circumsporozoite protein in sporozoite motility and infectivity, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 277, Pages: 47613-47618, ISSN: 0021-9258
- Author Web Link
- Cite
- Citations: 94
Bacarese-Hamilton T, Bistoni F, Crisanti A, 2002, Protein microarrays: From serodiagnosis to whole proteome scale analysis of the immune response against pathogenic microorganisms, BIOTECHNIQUES, Pages: 24-29, ISSN: 0736-6205
- Author Web Link
- Cite
- Citations: 29
Alphey L, Beard CB, Billingsley P, et al., 2002, Malaria control with genetically manipulated insect vectors, SCIENCE, Vol: 298, Pages: 119-121, ISSN: 0036-8075
- Author Web Link
- Cite
- Citations: 186
Bacarese-Hamilton T, Mezzasoma L, Ingham C, et al., 2002, Detection of allergen-specific IgE on microarrays by use of signal amplification techniques, CLINICAL CHEMISTRY, Vol: 48, Pages: 1367-1370, ISSN: 0009-9147
- Author Web Link
- Cite
- Citations: 49
Opitz C, Di Cristina M, Reiss M, et al., 2002, Intramembrane cleavage of microneme proteins at the surface of the apicomplexan parasite <i>Toxoplasma gondii</i>, EMBO JOURNAL, Vol: 21, Pages: 1577-1585, ISSN: 0261-4189
- Author Web Link
- Cite
- Citations: 96
Crisanti A, Ritort F, 2002, Inherent structures, configurational entropy and slow glassy dynamics, Workshop on Glassy Behaviour of Kinetically Constrained Models, Publisher: IOP PUBLISHING LTD, Pages: 1381-1395, ISSN: 0953-8984
- Author Web Link
- Cite
- Citations: 13
Crisanti A, Ritort F, Rocco A, et al., 2002, Is the Stillinger and Weber decomposition relevant for coarsening models?, Workshop on Glassy Behaviour of Kinetically Constrained Models, Publisher: IOP PUBLISHING LTD, Pages: 1523-1537, ISSN: 0953-8984
- Author Web Link
- Cite
- Citations: 5
Spano F, Ricci I, Di Cristina M, et al., 2002, The <i>SAG5</i> locus of <i>Toxoplasma gondii</i> encodes three novel proteins belonging to the SAG1 family of surface antigens, INTERNATIONAL JOURNAL FOR PARASITOLOGY, Vol: 32, Pages: 121-131, ISSN: 0020-7519
- Author Web Link
- Cite
- Citations: 13
Mezzasoma L, Bacarese-Hamilton T, Di Cristina M, et al., 2002, Antigen microarrays for serodiagnosis of infectious diseases, CLINICAL CHEMISTRY, Vol: 48, Pages: 121-130, ISSN: 0009-9147
- Author Web Link
- Cite
- Citations: 168
Crisanti A, Ritort F, 2002, A glass transition scenario based on heterogeneities and entropy barriers, 8th International Workshop on Disordered Systems, Publisher: TAYLOR & FRANCIS LTD, Pages: 143-149, ISSN: 0141-8637
- Author Web Link
- Cite
- Citations: 10
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.