Publications

BibTex format

@unpublished{Ledger:2017:10.1101/149971,
author = {Ledger, E and Pader, V and Edwards, A},
doi = {10.1101/149971},
publisher = {Microbiology Society},
title = {Enterococcus faecalis and pathogenic streptococci inactivate daptomycin by releasing phospholipids},
url = {http://dx.doi.org/10.1101/149971},
year = {2017}
}

Download

RIS format (EndNote, RefMan)

TY  - UNPB
AB  - Daptomycin is a lipopeptide antibiotic with activity against Gram-positive bacteria. We have shown previously that Staphylococcus aureus can survive daptomycin exposure by releasing membrane phospholipids that inactivate the antibiotic. To determine whether other pathogens possess this defence mechanism, phospholipid release and daptomycin activity were measured after incubation of Staphylococcus epidermidis, Group A or B streptococci, Streptococcus gordonii or Enterococcus faecalis with the antibiotic. All bacteria released phospholipid in response to daptomycin, which resulted in at least partial inactivation of the antibiotic. However, E. faecalis showed the highest levels of lipid release and daptomycin inactivation. As shown previously for S. aureus, phospholipid release by E. faecalis was inhibited by the lipid biosynthesis inhibitor platensimycin. In conclusion, several pathogenic Gram-positive bacteria, including E. faecalis, inactivate daptomycin by releasing phospholipids, which may contribute to the failure of daptomycin to resolve infections caused by these pathogens.
AU  - Ledger,E
AU  - Pader,V
AU  - Edwards,A
DO  - 10.1101/149971
PB  - Microbiology Society
PY  - 2017///
TI  - Enterococcus faecalis and pathogenic streptococci inactivate daptomycin by releasing phospholipids
UR  - http://dx.doi.org/10.1101/149971
ER  -

Download

DrAndrewEdwards

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)