Imperial College London
Portrait Picture

DrAndrewEdwards

Faculty of Medicine, Department of Infectious Disease

Director of Postgraduate Education & Senior Lecturer
 
 
 
//

Contact

 

a.edwards Website

 
 
//

Location

 

5.40AFlowers buildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Edwards:2011:10.3791/2693,
author = {Edwards, AM and Massey, RC},
doi = {10.3791/2693},
journal = {Journal of Visualized Experiments},
pages = {1--4},
title = {Invasion of human cells by a bacterial pathogen},
url = {http://dx.doi.org/10.3791/2693},
volume = {49},
year = {2011}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Here we will describe how we study the invasion of human endothelial cells by bacterial pathogen Staphylococcus aureus . The general protocol can be applied to the study of cell invasion by virtually any culturable bacterium. The stages at which specific aspects of invasion can be studied, such as the role of actin rearrangement or caveolae, will be highlighted. Host cells are grown in flasks and when ready for use are seeded into 24-well plates containing Thermanox coverslips. Using coverslips allows subsequent removal of the cells from the wells to reduce interference from serum proteins deposited onto the sides of the wells (to which S. aureus would attach). Bacteria are grown to the required density and washed to remove any secreted proteins (e.g. toxins). Coverslips with confluent layers of endothelial cells are transferred to new 24-well plates containing fresh culture medium before the addition of bacteria. Bacteria and cells are then incubated together for the required amount of time in 5% CO(2) at 37 degrees C. For S. aureus this is typically between 15-90 minutes. Thermanox coverslips are removed from each well and dip-washed in PBS to remove unattached bacteria. If total associated bacteria (adherent and internalised) are to be quantified, coverslips are then placed in a fresh well containing 0.5% Triton X-100 in PBS. Gentle pipetting leads to complete cell lysis and bacteria are enumerated by serial dilution and plating onto agar. If the number of bacteria that have invaded the cells is needed, coverslips are added to wells containing 500 mul tissue culture medium supplemented with gentamicin and incubation continued for 1 h, which will kill all external bacteria. Coverslips can then be washed, cells lysed and bacteria enumerated by plating onto agar as described above. If the experiment requires direct visualisation, coverslips can be fixed and stained for light, fluorescence or confocal microscopy or prepared for electron microscopy
AU  - Edwards,AM
AU  - Massey,RC
DO  - 10.3791/2693
EP  - 4
PY  - 2011///
SN  - 1940-087X
SP  - 1
TI  - Invasion of human cells by a bacterial pathogen
T2  - Journal of Visualized Experiments
UR  - http://dx.doi.org/10.3791/2693
UR  - pm:21445052
UR  - https://www.jove.com/t/2693/invasion-of-human-cells-by-a-bacterial-pathogen
UR  - http://hdl.handle.net/10044/1/83364
VL  - 49
ER  -
Download