Imperial College London
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ProfessorAlainFilloux

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 9651a.filloux Website CV

 
 
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Location

 

1.47Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wettstadt:2019:10.3389/fmicb.2019.01718,
author = {Wettstadt, S and Wood, TE and Fecht, S and Filloux, A},
doi = {10.3389/fmicb.2019.01718},
journal = {Frontiers in Microbiology},
pages = {1--18},
title = {Delivery of the Pseudomonas aeruginosa phospholipase effectors PldA and PldB in a VgrG- and H2-T6SS-dependent manner},
url = {http://dx.doi.org/10.3389/fmicb.2019.01718},
volume = {10},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The bacterial pathogen Pseudomonas aeruginosa uses three type VI secretion systems (T6SSs) to drive a multitude of effector proteins into eukaryotic or prokaryotic target cells. The T6SS is a supramolecular nanomachine, involving a set of 13 core proteins, which resembles the contractile tail of bacteriophages and whose tip is considered as a puncturing device helping to cross membranes. Effectors can attach directly to the T6SS spike which is composed of a VgrG (valine-glycine-rich proteins) trimer, of which P. aeruginosa produces several. We have previously shown that the master regulator RsmA controls the expression of all three T6SS gene clusters (H1-, H2- and H3-T6SS) and a range of remote vgrG and effector genes. We also demonstrated that specific interactions between VgrGs and various T6SS effectors are prerequisite for effector delivery in a process we called “à la carte delivery”. Here, we provide an in-depth description on how the two H2-T6SS-dependent effectors PldA and PldB are delivered via their cognate VgrGs, VgrG4b and VgrG5, respectively. We show that specific recognition of the VgrG C terminus is required and effector specificity can be swapped by exchanging these C-terminal domains. Importantly, we established that effector recognition by a cognate VgrG is not always sufficient to achieve successful secretion, but it is crucial to provide effector stability. This study highlights the complexity of effector adaptation to the T6SS nanomachine and shows how the VgrG tip can possibly be manipulated to achieve effector delivery.
AU  - Wettstadt,S
AU  - Wood,TE
AU  - Fecht,S
AU  - Filloux,A
DO  - 10.3389/fmicb.2019.01718
EP  - 18
PY  - 2019///
SN  - 1664-302X
SP  - 1
TI  - Delivery of the Pseudomonas aeruginosa phospholipase effectors PldA and PldB in a VgrG- and H2-T6SS-dependent manner
T2  - Frontiers in Microbiology
UR  - http://dx.doi.org/10.3389/fmicb.2019.01718
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2019.01718/full
UR  - http://hdl.handle.net/10044/1/72219
VL  - 10
ER  -
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