Imperial College London
Alternate

DrAmandaFoust

Faculty of Engineering, Department of Bioengineering

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 1055a.foust Website CV

 
 
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Location

 

RSM 4.05Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Foust:2015:10.1117/1.NPh.2.2.021007,
author = {Foust, AJ and Zampini, V and Tanese, D and Papagiakoumou, E and Emiliani, V},
doi = {10.1117/1.NPh.2.2.021007},
journal = {Neurophotonics},
pages = {021007--1--021007--8},
title = {Computer-generated holography enhances voltage dye fluorescence discrimination in adjacent neuronal structures},
url = {http://dx.doi.org/10.1117/1.NPh.2.2.021007},
volume = {2},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Voltage-sensitive fluorescence indicators enable tracking neuronal electrical signals simultaneously in multiple neurons or neuronal subcompartments difficult to access with patch electrodes. However, efficient widefield epifluorescence detection of rapid voltage fluorescence transients necessitates that imaged cells and structures lie sufficiently far from other labeled structures to avoid contamination from out of focal plane and scattered light. We overcame this limitation by exciting dye fluorescence with one-photon computer-generated holography shapes contoured to axons or dendrites of interest, enabling widefield detection of voltage fluorescence with high spatial specificity. By shaping light onto neighboring axons and dendrites, we observed that dendritic back-propagating action potentials were broader and slowly rising compared with axonal action potentials, differences not measured in the same structures illuminated with a large “pseudowidefield” (pWF) spot of the same excitation density. Shaped illumination trials showed reduced baseline fluorescence, higher baseline noise, and fractional fluorescence transient amplitudes two times greater than trials acquired with pWF illumination of the same regions.
AU  - Foust,AJ
AU  - Zampini,V
AU  - Tanese,D
AU  - Papagiakoumou,E
AU  - Emiliani,V
DO  - 10.1117/1.NPh.2.2.021007
EP  - 1
PY  - 2015///
SN  - 2329-4248
SP  - 021007
TI  - Computer-generated holography enhances voltage dye fluorescence discrimination in adjacent neuronal structures
T2  - Neurophotonics
UR  - http://dx.doi.org/10.1117/1.NPh.2.2.021007
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000370538400010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.spiedigitallibrary.org/journals/neurophotonics/volume-2/issue-02/021007/Computer-generated-holography-enhances-voltage-dye-fluorescence-discrimination-in-adjacent/10.1117/1.NPh.2.2.021007.full
VL  - 2
ER  -
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