Imperial College London
Alternate

Professor Angelika Gründling

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 5256a.grundling Website

 
 
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Location

 

6.22Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Karinou:2018:10.1101/311282,
author = {Karinou, E and Schuster, CF and Pazos, M and Vollmer, W and Gründling, A},
doi = {10.1101/311282},
title = {Inactivation of the monofunctional peptidoglycan glycosyltransferase SgtB allows<i>Staphylococcus aureus</i>to survive in the absence of lipoteichoic acid},
url = {http://dx.doi.org/10.1101/311282},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>The cell wall of<jats:italic>Staphylococcus aureus</jats:italic>is composed of peptidoglycan and the anionic polymers lipoteichoic acid (LTA) and wall teichoic acid. LTA is required for growth and normal cell morphology in<jats:italic>S. aureus.</jats:italic>Strains lacking LTA are usually only viable when grown under osmotically stabilizing conditions or after the acquisition of compensatory mutations. LTA negative suppressor strains with inactivating mutations in<jats:italic>gdpP</jats:italic>, resulting in an increase in intracellular c-di-AMP levels, have been described previously. Here, we sought to identify factors other than c-di-AMP that allow<jats:italic>S. aureus</jats:italic>to survive without LTA. LTA-negative strains able to grow in un-supplemented medium were obtained and found to contain mutations in<jats:italic>sgtB, mazE, clpX</jats:italic>or<jats:italic>vraT</jats:italic>. The growth improvement through mutations in<jats:italic>mazE</jats:italic>and<jats:italic>sgtB</jats:italic>was confirmed by complementation analysis. We also show that an<jats:italic>S. aureus sgtB</jats:italic>transposon mutant, inactivated for the monofunctional peptidoglycan glycosyltransferase SgtB, displays a 4-fold increase in the MIC towards a number of cell wall-targeting antibiotics, suggesting that alteration in the peptidoglycan structure could help bacteria compensate for the lack of LTA. Muropeptide analysis of peptidoglycan isolated from a WT and<jats:italic>sgtB</jats:italic>mutant strains did not reveal any sizable alternations in the peptidoglycan structure. In contrast, the peptidoglycan isolated from an LTA-negative<jats:italic>ltaS</jats:italic>mutant strain showed a significant reduction in the fraction of highly crosslinked peptidoglycan, which was partially rescued in
AU  - Karinou,E
AU  - Schuster,CF
AU  - Pazos,M
AU  - Vollmer,W
AU  - Gründling,A
DO  - 10.1101/311282
PY  - 2018///
TI  - Inactivation of the monofunctional peptidoglycan glycosyltransferase SgtB allows<i>Staphylococcus aureus</i>to survive in the absence of lipoteichoic acid
UR  - http://dx.doi.org/10.1101/311282
ER  -
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