Imperial College London
Alternate

Professor Angelika Gründling

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 5256a.grundling Website

 
 
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Location

 

6.22Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Rismondo:2019:10.1101/589911,
author = {Rismondo, J and Halbedel, S and Gründling, A},
doi = {10.1101/589911},
title = {Cell shape and antibiotic resistance is maintained by the activity of multiple FtsW and RodA enzymes in<i>Listeria monocytogenes</i>},
url = {http://dx.doi.org/10.1101/589911},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>Rod-shaped bacteria have two modes of peptidoglycan synthesis: lateral synthesis and synthesis at the cell division site. These two processes are controlled by two macromolecular protein complexes, the elongasome and divisome. Recently, it has been shown that the<jats:italic>Bacillus subtilis</jats:italic>RodA protein, which forms part of the elongasome, has peptidoglycan glycosyltransferase activity. The cell division specific RodA homolog FtsW fulfils a similar role at the divisome. The human pathogen<jats:italic>Listeria monocytogenes</jats:italic>encodes up to six FtsW/RodA homologs, however their functions have not yet been investigated. Analysis of deletion and depletion strains led to the identification of the essential cell division-specific FtsW protein, FtsW1. Interestingly,<jats:italic>L. monocytogenes</jats:italic>encodes a second FtsW protein, FtsW2, which can compensate for the lack of FtsW1, when expressed from an inducible promoter.<jats:italic>L. monocytogenes</jats:italic>also possesses three RodA homologs, RodA1, RodA2 and RodA3 and their combined absence is lethal. Cells of a<jats:italic>rodA1/rodA3</jats:italic>double mutant are shorter and have increased antibiotic and lysozyme sensitivity, probably due to a weakened cell wall. Results from promoter activity assays revealed that expression of<jats:italic>rodA3</jats:italic>and<jats:italic>ftsW2</jats:italic>is induced in the presence of antibiotics targeting penicillin binding proteins. Consistent with this, a<jats:italic>rodA3</jats:italic>mutant was more susceptible to the β-lactam antibiotic cefuroxime. Interestingly, overexpression of RodA3 also led to increased cefuroxime sensitivity. Our study highlights that<jats:italic>L. monocytogenes</jats:italic>encodes a multitude of functional FtsW and RodA enzymes to produce its
AU  - Rismondo,J
AU  - Halbedel,S
AU  - Gründling,A
DO  - 10.1101/589911
PY  - 2019///
TI  - Cell shape and antibiotic resistance is maintained by the activity of multiple FtsW and RodA enzymes in<i>Listeria monocytogenes</i>
UR  - http://dx.doi.org/10.1101/589911
ER  -
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