Imperial College London
Alternate

Professor Angelika Gründling

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 5256a.grundling Website

 
 
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Location

 

6.22Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zeden:2019:10.1101/754309,
author = {Zeden, MS and Kviatkovski, I and Schuster, CF and Thomas, VC and Fey, PD and Gründling, A},
doi = {10.1101/754309},
title = {Identification of the main glutamine and glutamate transporters in<i>Staphylococcus aureus</i>and their impact on c-di-AMP production},
url = {http://dx.doi.org/10.1101/754309},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Summary</jats:title><jats:p>A<jats:italic>Staphylococcus aureus</jats:italic>strain deleted for the c-di-AMP cyclase gene<jats:italic>dacA</jats:italic>is unable to survive in rich medium unless it acquires compensatory mutations. Previously identified mutations were in<jats:italic>opuD</jats:italic>, encoding the main glycine-betaine transporter, and<jats:italic>alsT</jats:italic>, encoding a predicted amino acid transporter. Here, we show that inactivation of OpuD restores the cell size of a<jats:italic>dacA</jats:italic>mutant to near wild-type size, while inactivation of AlsT does not, suggesting two different mechanisms for the growth rescue. AlsT was identified as an efficient glutamine transporter, indicating that preventing glutamine uptake in rich medium rescues the growth of the<jats:italic>S. aureus dacA</jats:italic>mutant. In addition, GltS was identified as a glutamine transporter. By performing growth curves with WT,<jats:italic>alsT</jats:italic>and<jats:italic>gltS</jats:italic>mutant strains in defined medium supplemented with ammonium, glutamine or glutamate, we revealed that ammonium and glutamine, but not glutamate promote the growth of<jats:italic>S. aureus</jats:italic>. This suggests that besides ammonium also glutamine can serve as a nitrogen source under these conditions. Ammonium and uptake of glutamine via AlsT inhibited c-di-AMP production, while glutamate uptake had no effect. These findings provide, besides the previously reported link between potassium and osmolyte uptake, a connection between nitrogen metabolism and c-di-AMP signalling in<jats:italic>S. aureus</jats:italic>.</jats:p><jats:sec><jats:title>Graphical abstract</jats:title><jats:fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><j
AU  - Zeden,MS
AU  - Kviatkovski,I
AU  - Schuster,CF
AU  - Thomas,VC
AU  - Fey,PD
AU  - Gründling,A
DO  - 10.1101/754309
PY  - 2019///
TI  - Identification of the main glutamine and glutamate transporters in<i>Staphylococcus aureus</i>and their impact on c-di-AMP production
UR  - http://dx.doi.org/10.1101/754309
ER  -
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