Imperial College London
Alternate

Professor Angelika Gründling

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 5256a.grundling Website

 
 
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Location

 

6.22Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sumrall:2020:10.1111/mmi.14455,
author = {Sumrall, ET and Schefer, CRE and Rismondo, J and Grundling, A and Boulos, S and Schneider, SR and Loessner, MJ and Shen, Y},
doi = {10.1111/mmi.14455},
journal = {Molecular Microbiology},
pages = {638--649},
title = {Galactosylated wall-teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes},
url = {http://dx.doi.org/10.1111/mmi.14455},
volume = {113},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Listeria  monocytogenes  is  a  Gram-positive,  intracellular  pathogen  harboring  the  surface-associated virulence factor InlB, which enables entry into certain host cells. Structurally diverse wall-teichoic  acids  (WTAs),  which  can  also  be  differentially  glycosylated,  determine  the antigenic basis of the various Listeria serovars. WTAs have many physiological functions; they can  serve  as  receptors  for  bacteriophages,  and  provide  a  substrate  for  binding  of  surface proteins  such  as  InlB.  In  contrast,  the  membrane-anchored  lipoteichoic  acids  (LTAs)  do  not show  significant  variation  and  do  not  contribute  to  serovar  determination.  It  was  previously demonstrated  that  surface-associated  InlB  non-covalently  adheres  to  both  WTA  and  LTA, mediating its retention on the cell wall. Here, we demonstrate that in a highly virulent serovar 4b  strain,  two  genes gtlB  and gttB are  responsible  for  galactosylation  of  LTA  and  WTA, respectively.  We  evaluated  the  InlB  surface  retention  in  mutants  lacking  each  of  these  two genes,  and  found  that  only  galactosylated  WTA  is  required  for  InlB  surface  presentation  and function, cellular invasiveness, and phage adsorption, while galactosylated LTA plays no role thereof.  Our  findings  demonstrate  that  a  simple  pathogen-defining  serovar  antigen,  that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.
AU  - Sumrall,ET
AU  - Schefer,CRE
AU  - Rismondo,J
AU  - Grundling,A
AU  - Boulos,S
AU  - Schneider,SR
AU  - Loessner,MJ
AU  - Shen,Y
DO  - 10.1111/mmi.14455
EP  - 649
PY  - 2020///
SN  - 0950-382X
SP  - 638
TI  - Galactosylated wall-teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/mmi.14455
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/mmi.14455
UR  - http://hdl.handle.net/10044/1/76880
VL  - 113
ER  -
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