Imperial College London
Alternate

Professor Angelika Gründling

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 5256a.grundling Website

 
 
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Location

 

6.22Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zeden:2020:10.1111/mmi.14479,
author = {Zeden, MS and Kviatkovski, I and Schuster, CF and Thomas, VC and Fey, PD and Grundling, A},
doi = {10.1111/mmi.14479},
journal = {Molecular Microbiology},
pages = {1085--1100},
title = {Identification of the main glutamine and glutamate transporters in Staphylococcus aureus and their impact on c-di-AMP production},
url = {http://dx.doi.org/10.1111/mmi.14479},
volume = {113},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A Staphylococcus aureus strain deleted for the cdiAMP cyclase gene dacA is unable to survive in rich medium unless it acquires compensatory mutations. Previously identified mutations were in opuD, encoding the main glycinebetaine transporter, and alsT, encoding a predicted amino acid transporter. Here, we show that inactivation of OpuD restores the cell size of a dacA mutant to near wildtype (WT) size, while inactivation of AlsT does not. AlsT was identified as an efficient glutamine transporter, indicating that preventing glutamine uptake in rich medium rescues the growth of the S. aureus dacA mutant. In addition, GltS was identified as a glutamate transporter. By performing growth curves with WT, alsT and gltS mutant strains in defined medium supplemented with ammonium, glutamine or glutamate, we revealed that ammonium and glutamine, but not glutamate promote the growth of S. aureus. This suggests that besides ammonium also glutamine can serve as a nitrogen source under these conditions. Ammonium and uptake of glutamine via AlsT and hence likely a higher intracellular glutamine concentration inhibited cdiAMP production, while glutamate uptake had no effect. These findings provide, besides the previously reported link between potassium and osmolyte uptake, a connection between nitrogen metabolism and cdiAMP signalling in S. aureus.
AU  - Zeden,MS
AU  - Kviatkovski,I
AU  - Schuster,CF
AU  - Thomas,VC
AU  - Fey,PD
AU  - Grundling,A
DO  - 10.1111/mmi.14479
EP  - 1100
PY  - 2020///
SN  - 0950-382X
SP  - 1085
TI  - Identification of the main glutamine and glutamate transporters in Staphylococcus aureus and their impact on c-di-AMP production
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/mmi.14479
UR  - http://hdl.handle.net/10044/1/77314
VL  - 113
ER  -
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