Imperial College London
Alternate

Professor Angelika Gründling

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 5256a.grundling Website

 
 
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Location

 

6.22Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rismondo:2018:10.1074/jbc.RA117.001614,
author = {Rismondo, J and Percy, MG and Gründling, A},
doi = {10.1074/jbc.RA117.001614},
journal = {J Biol Chem},
pages = {3293--3306},
title = {Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.},
url = {http://dx.doi.org/10.1074/jbc.RA117.001614},
volume = {293},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The bacterial cell wall is an important and highly complex structure that is essential for bacterial growth because it protects bacteria from cell lysis and environmental insults. A typical Gram-positive bacterial cell wall is composed of peptidoglycan and the secondary cell wall polymers, wall teichoic acid (WTA) and lipoteichoic acid (LTA). In many Gram-positive bacteria, LTA is a polyglycerol-phosphate chain that is decorated with d-alanine and sugar residues. However, the function of and proteins responsible for the glycosylation of LTA are either unknown or not well-characterized. Here, using bioinformatics, genetic, and NMR spectroscopy approaches, we found that the Bacillus subtilis csbB and yfhO genes are essential for LTA glycosylation. Interestingly, the Listeria monocytogenes gene lmo1079, which encodes a YfhO homolog, was not required for LTA glycosylation, but instead was essential for WTA glycosylation. LTA is polymerized on the outside of the cell and hence can only be glycosylated extracellularly. Based on the similarity of the genes coding for YfhO homologs that are required in B. subtilis for LTA glycosylation or in L. monocytogenes for WTA glycosylation, we hypothesize that WTA glycosylation might also occur extracellularly in Listeria species. Finally, we discovered that in L. monocytogenes, lmo0626 (gtlB) was required for LTA glycosylation, indicating that the encoded protein has a function similar to that of YfhO, although the proteins are not homologous. Together, our results enable us to propose an updated model for LTA glycosylation and also indicate that glycosylation of WTA might occur through two different mechanisms in Gram-positive bacteria.
AU  - Rismondo,J
AU  - Percy,MG
AU  - Gründling,A
DO  - 10.1074/jbc.RA117.001614
EP  - 3306
PY  - 2018///
SP  - 3293
TI  - Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation.
T2  - J Biol Chem
UR  - http://dx.doi.org/10.1074/jbc.RA117.001614
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29343515
UR  - http://hdl.handle.net/10044/1/56702
VL  - 293
ER  -
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