Imperial College London


Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Reader in Cell Biology



+44 (0)20 7594 2128a.hanyaloglu Website




Miss Kiran Dosanjh +44 (0)20 7594 2176




2009Institute of Reproductive and Developmental BiologyHammersmith Campus






BibTex format

author = {Jonas, KC and Hanyaloglu, AC},
booktitle = {Receptor-receptor interactions in the central nervous system},
doi = {10.1007/978-1-4939-8576-0_21},
pages = {329--343},
publisher = {Springer},
title = {Super-resolution imaging as a method to Study GPCR dimers and higher-order oligomers},
url = {},
year = {2018}

RIS format (EndNote, RefMan)

AB - The study of G protein-coupled receptor (GPCR) dimers and higher order oligomershas unveiled mechanisms for receptors to diversify signaling and potentially uncovernovel therapeutic targets. The functional and clinical significance of these receptorreceptor associations has been facilitated by the development of techniques andprotocols, enabling researchers to unpick their function from the molecularinterfaces, to demonstrating functional significance in vivo, in both health anddisease. Here we describe our methodology to study GPCR oligomerization at thesingle molecule level via super-resolution imaging. Specifically, we have employedphotoactivated localization microscopy, with photoactivatable dyes (PD-PALM) tovisualize the spatial organization of these complexes to <10nm resolution, and thequantitation of GPCR monomer, dimer and oligomer in both homomeric andheteromeric forms. We provide guidelines on optimal sample preparation, imagingparameters and necessary controls for resolving and quantifying single moleculedata. Finally, we discuss advantages and limitations of this imaging technique and itspotential future applications to the study of GPCR function.
AU - Jonas,KC
AU - Hanyaloglu,AC
DO - 10.1007/978-1-4939-8576-0_21
EP - 343
PB - Springer
PY - 2018///
SP - 329
TI - Super-resolution imaging as a method to Study GPCR dimers and higher-order oligomers
T1 - Receptor-receptor interactions in the central nervous system
UR -
UR -
ER -