Imperial College London

DrAylinHanyaloglu

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Reader in Cell Biology
 
 
 
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Contact

 

+44 (0)20 7594 2128a.hanyaloglu Website

 
 
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Assistant

 

Miss Kiran Dosanjh +44 (0)20 7594 2176

 
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Location

 

2009Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Owens:2019:10.1210/jc.2019-00780,
author = {Owens, LA and Kristensen, SG and Lerner, A and Christopoulos, G and Lavery, S and Hanyaloglu, AC and Hardy, K and Yding, Andersen C and Franks, S},
doi = {10.1210/jc.2019-00780},
journal = {Journal of Clinical Endocrinology and Metabolism},
pages = {6182--6192},
title = {Gene expression in granulosa cells from small antral follicles from women with or without polycystic ovaries},
url = {http://dx.doi.org/10.1210/jc.2019-00780},
volume = {104},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - CONTEXT: Polycystic ovary syndrome (PCOS) is the commonest cause of anovulation. A key feature of PCOS is arrest of follicles at the small-medium sized antral stage. OBJECTIVE AND DESIGN: To provide further insight into the mechanism of follicle arrest in PCOS, we profiled; (1) gonadotropin receptors; (2) characteristics of aberrant steroidogenesis, and (3) expression of anti-Mullerian hormone (AMH) and its receptor in granulosa cells (GCs) from unstimulated, human small antral follicles (hSAFs) and from granulosa-lutein cells (GLCs). SETTING: GCs from hSAFs were collected at the time of cryopreservation of ovarian tissue for fertility preservation and GLCs collected during oocyte aspiration before IVF/ICSI. PARTICIPANTS: hSAF GCs were collected from 31 women (98 follicles), 10 with polycystic ovaries (PCO) and 21 without. GLCs were collected from 6 women with PCOS and 6 controls undergoing IVF. MAIN OUTCOME MEASURES: Expression of the following genes: LHCGR, FSHR, AR, INSR, HSD3B2, CYP11A1, CYP19, STAR, AMH, AMHR2, FST, INHBA, INHBB in GCs and GLCs were compared between women with PCO and controls. RESULTS: GCs in hSAFs from PCO women showed higher expression of LHCGR in a subset (20%) of follicles. Expression of FSHR (p<0.05), AR (p<0.05), CYP11A1 (P<0.05) was lower, and expression of CYP19A1 (p<0.05), STAR (p<0.05), HSD3B2 (ns), INHBA (p<0.05) higher in PCO GCs. Gene expression in GL cells differed between women with and without PCOS but also differed from that in GCs. CONCLUSIONS: Follicle arrest in PCO is characterised in GCs by differential regulation of key genes involved in follicle growth and function.
AU - Owens,LA
AU - Kristensen,SG
AU - Lerner,A
AU - Christopoulos,G
AU - Lavery,S
AU - Hanyaloglu,AC
AU - Hardy,K
AU - Yding,Andersen C
AU - Franks,S
DO - 10.1210/jc.2019-00780
EP - 6192
PY - 2019///
SN - 0021-972X
SP - 6182
TI - Gene expression in granulosa cells from small antral follicles from women with or without polycystic ovaries
T2 - Journal of Clinical Endocrinology and Metabolism
UR - http://dx.doi.org/10.1210/jc.2019-00780
UR - https://www.ncbi.nlm.nih.gov/pubmed/31276164
UR - https://academic.oup.com/jcem/advance-article/doi/10.1210/jc.2019-00780/5528233
UR - http://hdl.handle.net/10044/1/71771
VL - 104
ER -