Imperial College London


Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Reader in Cell Biology



+44 (0)20 7594 2128a.hanyaloglu Website




Miss Kiran Dosanjh +44 (0)20 7594 2176




2009Institute of Reproductive and Developmental BiologyHammersmith Campus






BibTex format

author = {Owens, LA and Abbara, A and Lerner, A and O'floinn, S and Christopoulos, G and Khanjani, S and Islam, R and Hardy, K and Hanyaloglu, AC and Lavery, SA and Dhillo, WS and Franks, S},
doi = {humrep/dex357},
journal = {Human Reproduction},
pages = {292--302},
title = {The direct and indirect effects of kisspeptin-54 on granulosa lutein cell function},
url = {},
volume = {33},
year = {2018}

RIS format (EndNote, RefMan)

AB - STUDY QUESTIONWhat are the in vivo and in vitro actions of kisspeptin-54 on the expression of genes involved in ovarian reproductive function, steroidogenesis and ovarian hyperstimulation syndrome (OHSS) in granulosa lutein (GL) cells when compared with traditional triggers of oocyte maturation?SUMMARY ANSWERThe use of kisspeptin-54 as an oocyte maturation trigger augmented expression of genes involved in ovarian steroidogenesis in human GL cells including, FSH receptor (FSHR), LH/hCG receptor (LHCGR), steroid acute regulatory protein (STAR), aromatase, estrogen receptors alpha and beta (ESR1, ESR2), 3-beta-hydroxysteroid dehydrogenase type 2 (3BHSD2) and inhibin A (INHBA), when compared to traditional maturation triggers, but did not alter markers of OHSS.WHAT IS KNOWN ALREADYhCG is the most widely used trigger of oocyte maturation, but is associated with an increased risk of OHSS. The use of GnRH agonists to trigger oocyte maturation is a safer alternative to hCG. More recently, kisspeptin-54 has emerged as a novel therapeutic option that safely triggers oocyte maturation even in women at high risk of OHSS. Kisspeptin indirectly stimulates gonadotropin secretion by acting on hypothalamic GnRH neurons. Kisspeptin and its receptor are also expressed in the human ovary, but there is limited data on the direct action of kisspeptin on the ovary.STUDY DESIGN SIZE, DURATIONForty-eight women undergoing IVF treatment for infertility consented to kisspeptin-54 triggering and/or granulosa cell collection and were included in the study. Twelve women received hCG, 12 received GnRH agonist and 24 received kisspeptin-54 to trigger oocyte maturation. In the kisspeptin-54 group, 12 received one injection of kisseptin-54 (9.6 nmol/kg) and 12 received two injections of kisspeptin-54 at a 10 h interval (9.6 nmol/kg × 2).PARTICIPANTS/MATERIALS, SETTING, METHODSFollicular fluid was aspirated and pooled from follicles during the retrieval of oocytes for IVF/ICSI. GL cells were iso
AU - Owens,LA
AU - Abbara,A
AU - Lerner,A
AU - O'floinn,S
AU - Christopoulos,G
AU - Khanjani,S
AU - Islam,R
AU - Hardy,K
AU - Hanyaloglu,AC
AU - Lavery,SA
AU - Dhillo,WS
AU - Franks,S
DO - humrep/dex357
EP - 302
PY - 2018///
SN - 1460-2350
SP - 292
TI - The direct and indirect effects of kisspeptin-54 on granulosa lutein cell function
T2 - Human Reproduction
UR -
UR -
VL - 33
ER -