238 results found
Buldum G, Bismarck A, Mantalaris A, 2018, Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli, BIOPROCESS AND BIOSYSTEMS ENGINEERING, Vol: 41, Pages: 265-279, ISSN: 1615-7591
Grilo AL, Mantalaris A, 2018, The Increasingly Human and Profitable Monoclonal Antibody Market., Trends Biotechnol
The monoclonal antibody (mAb) market has changed rapidly in the past 5 years: it has doubled in size, becoming dominated by fully human molecules, launched bispecific molecules, and faced competition from biosimilars. We summarize the market in terms of therapeutic applications, type and structure of mAbs, dominant companies, manufacturing locations, and emerging markets.
Quiroga-Campano AL, Panoskaltsis N, Mantalaris A, 2018, Energy-based culture medium design for biomanufacturing optimization: A case study in monoclonal antibody production by GS-NS0 cells, METABOLIC ENGINEERING, Vol: 47, Pages: 21-30, ISSN: 1096-7176
Tsipa A, Koutinas M, Usaku C, et al., 2018, Optimal bioprocess design through a gene regulatory network - Growth kinetic hybrid model: Towards replacing Monod kinetics., Metab Eng, Vol: 48, Pages: 129-137
Currently, design and optimisation of biotechnological bioprocesses is performed either through exhaustive experimentation and/or with the use of empirical, unstructured growth kinetics models. Whereas, elaborate systems biology approaches have been recently explored, mixed-substrate utilisation is predominantly ignored despite its significance in enhancing bioprocess performance. Herein, bioprocess optimisation for an industrially-relevant bioremediation process involving a mixture of highly toxic substrates, m-xylene and toluene, was achieved through application of a novel experimental-modelling gene regulatory network - growth kinetic (GRN-GK) hybrid framework. The GRN model described the TOL and ortho-cleavage pathways in Pseudomonas putida mt-2 and captured the transcriptional kinetics expression patterns of the promoters. The GRN model informed the formulation of the growth kinetics model replacing the empirical and unstructured Monod kinetics. The GRN-GK framework's predictive capability and potential as a systematic optimal bioprocess design tool, was demonstrated by effectively predicting bioprocess performance, which was in agreement with experimental values, when compared to four commonly used models that deviated significantly from the experimental values. Significantly, a fed-batch biodegradation process was designed and optimised through the model-based control of TOL Pr promoter expression resulting in 61% and 60% enhanced pollutant removal and biomass formation, respectively, compared to the batch process. This provides strong evidence of model-based bioprocess optimisation at the gene level, rendering the GRN-GK framework as a novel and applicable approach to optimal bioprocess design. Finally, model analysis using global sensitivity analysis (GSA) suggests an alternative, systematic approach for model-driven strain modification for synthetic biology and metabolic engineering applications.
Kang Y, Georgiou AI, MacFarlane RJ, et al., 2017, Fibronectin stimulates the osteogenic differentiation of murine embryonic stem cells, JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol: 11, Pages: 1929-1940, ISSN: 1932-6254
Tsipa A, Koutinas M, Vernardis SI, et al., 2017, The impact of succinate trace on pWW0 and ortho-cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation, BIORESOURCE TECHNOLOGY, Vol: 234, Pages: 397-405, ISSN: 0960-8524
Fauzi I, Panoskaltsis N, Mantalaris A, 2016, In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production., Methods Mol Biol, Vol: 1341, Pages: 217-234
Embryonic stem cells (ESCs) differentiation via embryoid body (EB) formation is an established method that generates the three germ layers. However, EB differentiation poses several problems including formation of heterogeneous cell populations. Herein, we described a differentiation protocol on enhancing mesoderm derivation from murine ESCs (mESCs) using conditioned medium (CM) from HepG2 cells. We used this technique to direct hematopoiesis by generating "embryoid-like" colonies (ELCs) from murine (m) ESCs without following standard formation of EBs. Our CM-mESCs group yielded an almost fivefold increase in ELC formation (p ≤ 0.05) and higher expression of mesoderm genes;-Brachyury-T, Goosecoid, and Flk-1 compared with control mESCs group. Hematopoietic colony formation from CM-mESCs was also enhanced by twofold at days 7 and 14 with earlier colony commitment compared to control mESCs (p ≤ 0.05). This early clonogenic capacity was confirmed morphologically by the presence of nucleated erythrocytes and macrophages as early as day 7 in culture using standard 14-day colony-forming assay. Early expression of hematopoietic primitive (ζ-globin) and definitive (β-globin) erythroid genes and proteins was also observed by day 7 in the CM-treated culture. These data indicate that hematopoietic cells more quickly differentiate from CM-treated, compared with those using standard EB approaches, and provide an efficient bioprocess platform for erythroid-specific differentiation of ESCs.
Klontzas ME, Kenanidis EI, MacFarlane RJ, et al., 2016, Investigational drugs for fracture healing: preclinical & clinical data, EXPERT OPINION ON INVESTIGATIONAL DRUGS, Vol: 25, Pages: 585-596, ISSN: 1354-3784
Kostoglou M, Fuentes-Gari M, Garcia-Munzer D, et al., 2016, A comprehensive mathematical analysis of a novel multistage population balance model for cell proliferation, COMPUTERS & CHEMICAL ENGINEERING, Vol: 91, Pages: 157-166, ISSN: 0098-1354
Papathanasiou MM, Avraamidou S, Oberdieck R, et al., 2016, Advanced Control Strategies for the Multicolumn Countercurrent Solvent Gradient Purification Process, AICHE JOURNAL, Vol: 62, Pages: 2341-2357, ISSN: 0001-1541
Reissis D, Quen OT, Cooper NC, et al., 2016, Current clinical evidence for the use of mesenchymal stem cells in articular cartilage repair, EXPERT OPINION ON BIOLOGICAL THERAPY, Vol: 16, Pages: 535-557, ISSN: 1471-2598
Savvopoulos S, Misener R, Panoskaltsis N, et al., 2016, A Personalized Framework for Dynamic Modeling of Disease Trajectories in Chronic Lymphocytic Leukemia, IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, Vol: 63, Pages: 2396-2404, ISSN: 0018-9294
Severn CE, Macedo H, Eagle MJ, et al., 2016, Polyurethane scaffolds seeded with CD34(+) cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322
Tsipa A, Koutinas M, Pistikopoulos EN, et al., 2016, Transcriptional kinetics of the cross-talk between the ortho-cleavage and TOL pathways of toluene biodegradation in Pseudomonas putida mt-2, JOURNAL OF BIOTECHNOLOGY, Vol: 228, Pages: 112-123, ISSN: 0168-1656
Zubairi SI, Mantalaris A, Bismarck A, et al., 2016, POLYHYDROXYALKANOATES (PHAs) FOR TISSUE ENGINEERING APPLICATIONS: BIOTRANSFORMATION OF PALM OIL MILL EFFLUENT (POME) TO VALUE-ADDED POLYMERS, JURNAL TEKNOLOGI, Vol: 78, Pages: 13-29, ISSN: 0127-9696
Allenby MC, Tahlawi A, Dos Santos SB, et al., 2015, Development of a Hematopoietic Microenvironment for the Production of Red Blood Cells (RBCs) in a Novel 3D Hollow Fibre Bioreactor, TISSUE ENGINEERING PART A, Vol: 21, Pages: S15-S16, ISSN: 1937-3341
Allenby MC, Tahlawi A, dos Santos SB, et al., 2015, DEVELOPMENT OF AN EX VIVO BONE MARROW MIMICRY MICROENVIRONMENT IN A NOVEL 3D HOLLOW FIBRE BIOREACTOR, 44th Annual Scientific Meeting of the International-Society-for-Experimental-Hematology (ISEH), Publisher: ELSEVIER SCIENCE INC, Pages: S51-S51, ISSN: 0301-472X
Fuentes-Gari M, Misener R, Garcia-Munzer D, et al., 2015, A mathematical model of subpopulation kinetics for the deconvolution of leukaemia heterogeneity, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 12, ISSN: 1742-5689
Fuentes-Gari M, Misener R, Georgiadis MC, et al., 2015, Selecting a Differential Equation Cell Cycle Model for Simulating Leukemia Treatment, INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, Vol: 54, Pages: 8847-8859, ISSN: 0888-5885
Fuentes-Gari M, Misener R, Pefani E, et al., 2015, Cell cycle model selection for leukemia and its impact in chemotherapy outcomes, 12TH INTERNATIONAL SYMPOSIUM ON PROCESS SYSTEMS ENGINEERING AND 25TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING, PT C, Vol: 37, Pages: 2159-2164, ISSN: 1570-7946
Fuentes-Gari M, Velliou E, Misener R, et al., 2015, A systematic framework for the design, simulation and optimization of personalized healthcare: Making and healing blood, COMPUTERS & CHEMICAL ENGINEERING, Vol: 81, Pages: 80-93, ISSN: 0098-1354
Fuentes-Gari M, Zemenides S, Misener R, et al., 2015, Use of Mathematical Modelling Indicates That Patients Treated for Acute Myeloid Leukaemia (AML) Are Undertreated When Ideal Body Weight Is Used to Dose Chemotherapy, 57th Annual Meeting of the American-Society-of-Hematology, Publisher: AMER SOC HEMATOLOGY, ISSN: 0006-4971
Fuentes-Gaŕ M, Misener R, Georgiadis MC, et al., 2015, Chemotherapy optimization in Leukemia: Selecting the right mathematical models for the right biological processes, Pages: 534-539, ISSN: 1474-6670
© 2015, IFAC (International Federation of Automatic Control) Hosting by Elsevier Ltd. All rights reserved. Clinical chemotherapy dosage strategies for leukemia rely on weight/height calculations theoretically correlated to patient drug tolerance. However, over-and under-dosage still exist in clinical practice, which could be overcome by quantifying the actual fraction of cancer cells susceptible to be eradicated. In this work, we show how choosing models that are accurate enough in simulating the biological processes ultimately affecting drug efficacy is critical in order to disentangle patient to patient heterogeneity. Incorporating heterogeneity from measurable sources in such a manner brings us a step closer in our path towards the development of personalized rational therapies.
Kiparissides A, Pistikopoulos EN, Mantalaris A, 2015, On the Model-Based Optimization of Secreting Mammalian Cell (GS-NS0) Cultures, BIOTECHNOLOGY AND BIOENGINEERING, Vol: 112, Pages: 536-548, ISSN: 0006-3592
Klontzas ME, Kenanidis EI, Heliotis M, et al., 2015, Bone and cartilage regeneration with the use of umbilical cord mesenchymal stem cells, EXPERT OPINION ON BIOLOGICAL THERAPY, Vol: 15, Pages: 1541-1552, ISSN: 1471-2598
Kostoglou M, Fuentes-Gari M, Garcia-Muenzer D, et al., 2015, Mathematical analysis of multistage population balances for cell growth and death, 12TH INTERNATIONAL SYMPOSIUM ON PROCESS SYSTEMS ENGINEERING AND 25TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING, PT C, Vol: 37, Pages: 2105-2110, ISSN: 1570-7946
Muenzer DGG, Ivarsson M, Usaku C, et al., 2015, An unstructured model of metabolic and temperature dependent cell cycle arrest in hybridoma batch and fed-batch cultures, BIOCHEMICAL ENGINEERING JOURNAL, Vol: 93, Pages: 260-273, ISSN: 1369-703X
Muenzer DGG, Kostoglou M, Georgiadis MC, et al., 2015, Cyclin and DNA Distributed Cell Cycle Model for GS-NS0 Cells, PLOS COMPUTATIONAL BIOLOGY, Vol: 11, ISSN: 1553-734X
Papathanasiou MM, Steinebach F, Stroehlein G, et al., 2015, A control strategy for periodic systems - application to the twin-column MCSGP, 12th International Symposium on Process Systems Engineering (PSE) / 25th European Symposium on Computer Aided Process Engineering (ESCAPE), Publisher: ELSEVIER SCIENCE BV, Pages: 1505-1510, ISSN: 1570-7946
Savvopoulos S, Misener R, Panoskaltsis N, et al., 2015, Global Sensitivity Analysis for a Model of B-Cell Chronic Lymphocytic Leukemia Disease Trajectories, 12TH INTERNATIONAL SYMPOSIUM ON PROCESS SYSTEMS ENGINEERING (PSE) AND 25TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING (ESCAPE), PT A, Vol: 37, Pages: 185-190, ISSN: 1570-7946
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