BibTex format
@article{Cuesta:2009,
author = {Cuesta, R and MartÃnez-Sanchez, A and Gebauer, F},
journal = {MOLECULAR AND CELLULAR BIOLOGY},
title = {miR-181a Regulates Cap-Dependent Translation of p27kip1mRNA in Myeloid Cells},
year = {2009}
}
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RIS format (EndNote, RefMan)
TY - JOUR
AB - p27kip1 (p27) is a cell cycle inhibitor and tumor suppressor whose expression is tightly regulated in the cell.Translational control of p27 mRNA has emerged as a prominent mechanism to regulate p27 expression duringdifferentiation, quiescence, and cancer progression. The microRNAs miR-221 and miR-222 repress p27 expressionin various cancer cells, and this repression promotes tumor cell proliferation. In addition, thepresence of an internal ribosome entry site in the 5 untranslated region (UTR) of p27 mRNA has beenreported. Here, we show that p27 mRNA is translated via a cap-dependent mechanism in HeLa and HL60 cellsand that the previously reported IRES activity can be attributed to cryptic promoters in the sequencecorresponding to the p27 5 UTR. Furthermore, cap-dependent translation of p27 mRNA is repressed bymiR-181a in undifferentiated HL60 cells. Repression by miR-181a is relieved during differentiation of HL60into monocyte-like cells, allowing the accumulation of p27, which is necessary to fully block cell cycle progressionand reach terminal differentiation. These results identify miR-181a as a regulator of p27 mRNA translationduring myeloid cell differentiation.
AU - Cuesta,R
AU - MartÃnez-Sanchez,A
AU - Gebauer,F
PY - 2009///
TI - miR-181a Regulates Cap-Dependent Translation of p27kip1mRNA in Myeloid Cells
T2 - MOLECULAR AND CELLULAR BIOLOGY
ER -