Imperial College London
Portrait Picture

ProfessorAnnaRandi

Faculty of Medicine, National Heart & Lung Institute

Head of Section for Vascular Science
 
 
 
//

Contact

 

a.randi Website

 
 
//

Location

 

L-block, room 533Hammersmith HospitalHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Perbellini:2017:cvr/cvx152,
author = {Perbellini, F and Watson, SA and Scigliano, M and Alayoubi, S and Tkach, S and Bardi, I and Quaife, N and Kane, C and Dufton, NP and Simon, A and Sikkel, MB and Faggian, G and Randi, AM and Gorelik, J and Harding, S and Terracciano, CMN},
doi = {cvr/cvx152},
journal = {Cardiovascular Research},
pages = {77--89},
title = {Investigation of cardiac fibroblasts using myocardial slices},
url = {http://dx.doi.org/10.1093/cvr/cvx152},
volume = {114},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - AimsCardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When isolated and cultured in vitro, CFs undergo rapid phenotypic changes including increased expression of α-SMA. Here we describe a new model to study CFs and their response to pharmacological and mechanical stimuli using in vitro cultured mouse, dog and human myocardial slices.Methods and resultsUnloading of myocardial slices induced CF proliferation without α-SMA expression up to 7 days in culture. CFs migrating onto the culture plastic support or cultured on glass expressed αSMA within 3 days. The cells on the slice remained αSMA(−) despite transforming growth factor-β (20 ng/ml) or angiotensin II (200 µM) stimulation. When diastolic load was applied to myocardial slices using A-shaped stretchers, CF proliferation was significantly prevented at Days 3 and 7 (P < 0.001).ConclusionsMyocardial slices allow the study of CFs in a multicellular environment and may be used to effectively study mechanisms of cardiac fibrosis and potential targets.
AU  - Perbellini,F
AU  - Watson,SA
AU  - Scigliano,M
AU  - Alayoubi,S
AU  - Tkach,S
AU  - Bardi,I
AU  - Quaife,N
AU  - Kane,C
AU  - Dufton,NP
AU  - Simon,A
AU  - Sikkel,MB
AU  - Faggian,G
AU  - Randi,AM
AU  - Gorelik,J
AU  - Harding,S
AU  - Terracciano,CMN
DO  - cvr/cvx152
EP  - 89
PY  - 2017///
SN  - 1755-3245
SP  - 77
TI  - Investigation of cardiac fibroblasts using myocardial slices
T2  - Cardiovascular Research
UR  - http://dx.doi.org/10.1093/cvr/cvx152
UR  - http://hdl.handle.net/10044/1/50360
VL  - 114
ER  -
Download